Use of the lipid emulsion and Samonella thyphimurium mitogen adjuvant to stimulate IgG production in chickens

C. McCune, R. H. Goddard, S. O. Cho, S. M. Wick

Research output: Contribution to journalArticlepeer-review

Abstract

Chickens were immunized with the lipid emulsion system and Salmonella typhimurium mitogen adjuvant for fowl plus synthetic peptides corresponding to the divergent amino acid sequences at the carboxyl terminus of each of two different α-tubulin isoforms from Arabidopis thaliana. Antibodies were extracted from egg yolks and used in immunoblot assay to determine the time course of antibody production in two chickens. Specific immunoglobulin production increased 10-14 days after the primary injection in both chickens and reached peak levels shortly thereafter. Booster injections helped maintain IgG production, which eventually dropped off after the final injection.

Original languageEnglish (US)
Pages (from-to)267-270
Number of pages4
JournalJournal of Immunological Methods
Volume155
Issue number2
DOIs
StatePublished - Nov 5 1992

Keywords

  • (Chicken)
  • Plant tubulin
  • Yolk IgG
  • Yolk antibody

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