Abstract
Double-stranded DNA is separated by means of capillary electrophoresis with either hydroxypropylmethyl cellulose or non-cross-linked polyacrylamide as the separation medium. A high-sensitivity laser-induced fluorescence detector is used to monitor the elution of DNA that has been stained with the intercalating dyes POPO-3, YOYO-3 or YOYO-1. Detection limits are on the order of 1 zmol of dye. The dye is incorporated at a rate of 1 dye molecule for every 10 base pairs in the double-stranded DNA; as a result, detection limits are a few yoctomoles of fluorescently labeled DNA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 205-216 |
| Number of pages | 12 |
| Journal | Journal of Chromatography A |
| Volume | 669 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - May 27 1994 |
Bibliographical note
Funding Information:This work was funded by the Natural Sciences and Engineering Research Council of Canada (NSERC). D.F. acknowledges a predoctoral fellowship from NSERC; N.J.D. acknowledgesa Steacie Fellowship from NSERC.
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