Use of the fluorescent intercalating dyes POPO-3, YOYO-3 and YOYO-1 for ultrasensitive detection of double-stranded DNA separated by capillary electrophoresis with hydroxypropylmethyl cellulose and non-cross-linked polyacrylamide

Daniel Figeys, Edgar Arriaga, Annika Renborg, Norman J. Dovichi

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

Double-stranded DNA is separated by means of capillary electrophoresis with either hydroxypropylmethyl cellulose or non-cross-linked polyacrylamide as the separation medium. A high-sensitivity laser-induced fluorescence detector is used to monitor the elution of DNA that has been stained with the intercalating dyes POPO-3, YOYO-3 or YOYO-1. Detection limits are on the order of 1 zmol of dye. The dye is incorporated at a rate of 1 dye molecule for every 10 base pairs in the double-stranded DNA; as a result, detection limits are a few yoctomoles of fluorescently labeled DNA.

Original languageEnglish (US)
Pages (from-to)205-216
Number of pages12
JournalJournal of Chromatography A
Volume669
Issue number1-2
DOIs
StatePublished - May 27 1994

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