Cross-linked, poly(N-isopropylacrylamide) gel was used to concentrate avian influenza virus from allantoic fluid. Placing the gel in virus-infected allantoic fluid at 4°C caused the gel to swell and absorb small molecular weight solutes, while excluding avian influenza virus and other large particles. Warming the gel to 37°C or more caused the gel to collapse. The gel remained functional after sterilization in an autoclave and could be reused to concentrate other samples of allantoic fluid. Using a combined concentration and elution technique, we were able to achieve an average of 84.2% virus recovery, while reducing the fluid volume from 90 ml to 7.6 ml.
Bibliographical noteFunding Information:
Thanks are due to Richard Peterson and Theresa Schneider for technical assistance and to Lori Rehnblom for typing the manuscript. This study was supported in part by grants from the U.S. Department of Agriculture (85-CRSR-2-2586) and from the Graduate School, University of Minnesota.
- Influenza virus
- Polyacrylamide gel
- Temperature-sensitive gel
- Virus concentration