Use of temperature-sensitive gel for concentration of influenza virus from infected allantoic fluids

Donald C. Roepke; Sagar M. Goyal; Charles J. Kelleher; David A. Halvorson; Albert J. Abraham; Roberto F.S. Freitas; Edward L. Cussler

doi:10.1016/0166-0934(87)90045-0

Use of temperature-sensitive gel for concentration of influenza virus from infected allantoic fluids

Donald C. Roepke, Sagar M. Goyal, Charles J. Kelleher, David A. Halvorson, Albert J. Abraham, Roberto F.S. Freitas, Edward L. Cussler

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Cross-linked, poly(N-isopropylacrylamide) gel was used to concentrate avian influenza virus from allantoic fluid. Placing the gel in virus-infected allantoic fluid at 4°C caused the gel to swell and absorb small molecular weight solutes, while excluding avian influenza virus and other large particles. Warming the gel to 37°C or more caused the gel to collapse. The gel remained functional after sterilization in an autoclave and could be reused to concentrate other samples of allantoic fluid. Using a combined concentration and elution technique, we were able to achieve an average of 84.2% virus recovery, while reducing the fluid volume from 90 ml to 7.6 ml.

Original language	English (US)
Pages (from-to)	25-31
Number of pages	7
Journal	Journal of Virological Methods
Volume	15
Issue number	1
DOIs	https://doi.org/10.1016/0166-0934(87)90045-0
State	Published - Jan 1987

Bibliographical note

Funding Information:
Thanks are due to Richard Peterson and Theresa Schneider for technical assistance and to Lori Rehnblom for typing the manuscript. This study was supported in part by grants from the U.S. Department of Agriculture (85-CRSR-2-2586) and from the Graduate School, University of Minnesota.

Keywords

Influenza virus
Polyacrylamide gel
Temperature-sensitive gel
Virus concentration

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0166-0934(87)90045-0

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title = "Use of temperature-sensitive gel for concentration of influenza virus from infected allantoic fluids",

abstract = "Cross-linked, poly(N-isopropylacrylamide) gel was used to concentrate avian influenza virus from allantoic fluid. Placing the gel in virus-infected allantoic fluid at 4°C caused the gel to swell and absorb small molecular weight solutes, while excluding avian influenza virus and other large particles. Warming the gel to 37°C or more caused the gel to collapse. The gel remained functional after sterilization in an autoclave and could be reused to concentrate other samples of allantoic fluid. Using a combined concentration and elution technique, we were able to achieve an average of 84.2% virus recovery, while reducing the fluid volume from 90 ml to 7.6 ml.",

keywords = "Influenza virus, Polyacrylamide gel, Temperature-sensitive gel, Virus concentration",

author = "Roepke, {Donald C.} and Goyal, {Sagar M.} and Kelleher, {Charles J.} and Halvorson, {David A.} and Abraham, {Albert J.} and Freitas, {Roberto F.S.} and Cussler, {Edward L.}",

note = "Funding Information: Thanks are due to Richard Peterson and Theresa Schneider for technical assistance and to Lori Rehnblom for typing the manuscript. This study was supported in part by grants from the U.S. Department of Agriculture (85-CRSR-2-2586) and from the Graduate School, University of Minnesota.",

year = "1987",

month = jan,

doi = "10.1016/0166-0934(87)90045-0",

language = "English (US)",

volume = "15",

pages = "25--31",

journal = "Journal of Virological Methods",

issn = "0166-0934",

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TY - JOUR

T1 - Use of temperature-sensitive gel for concentration of influenza virus from infected allantoic fluids

AU - Roepke, Donald C.

AU - Goyal, Sagar M.

AU - Kelleher, Charles J.

AU - Halvorson, David A.

AU - Abraham, Albert J.

AU - Freitas, Roberto F.S.

AU - Cussler, Edward L.

N1 - Funding Information: Thanks are due to Richard Peterson and Theresa Schneider for technical assistance and to Lori Rehnblom for typing the manuscript. This study was supported in part by grants from the U.S. Department of Agriculture (85-CRSR-2-2586) and from the Graduate School, University of Minnesota.

PY - 1987/1

Y1 - 1987/1

N2 - Cross-linked, poly(N-isopropylacrylamide) gel was used to concentrate avian influenza virus from allantoic fluid. Placing the gel in virus-infected allantoic fluid at 4°C caused the gel to swell and absorb small molecular weight solutes, while excluding avian influenza virus and other large particles. Warming the gel to 37°C or more caused the gel to collapse. The gel remained functional after sterilization in an autoclave and could be reused to concentrate other samples of allantoic fluid. Using a combined concentration and elution technique, we were able to achieve an average of 84.2% virus recovery, while reducing the fluid volume from 90 ml to 7.6 ml.

AB - Cross-linked, poly(N-isopropylacrylamide) gel was used to concentrate avian influenza virus from allantoic fluid. Placing the gel in virus-infected allantoic fluid at 4°C caused the gel to swell and absorb small molecular weight solutes, while excluding avian influenza virus and other large particles. Warming the gel to 37°C or more caused the gel to collapse. The gel remained functional after sterilization in an autoclave and could be reused to concentrate other samples of allantoic fluid. Using a combined concentration and elution technique, we were able to achieve an average of 84.2% virus recovery, while reducing the fluid volume from 90 ml to 7.6 ml.

KW - Influenza virus

KW - Polyacrylamide gel

KW - Temperature-sensitive gel

KW - Virus concentration

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U2 - 10.1016/0166-0934(87)90045-0

DO - 10.1016/0166-0934(87)90045-0

M3 - Article

C2 - 3805229

AN - SCOPUS:0023100125

SN - 0166-0934

VL - 15

SP - 25

EP - 31

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 1

ER -

Use of temperature-sensitive gel for concentration of influenza virus from infected allantoic fluids

Abstract

Bibliographical note

Keywords

UN SDGs

Publisher link

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