Use of Sodium Hydroxide DNA Extraction Methods for Nested PCR Detection of Bretziella fagacearum in the Sapwood of Oak Species in Minnesota

Melanie J. Moore, Jennifer Juzwik, Olga Saiapina, Snober Ahmed, Anna M Yang, Abdennour Abbas

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Oak wilt caused by Bretziella fagacearum is an important disease of Quercus spp.; however, its diagnosis may be confused with damage resulting from other diseases, insects, or abiotic factors. Laboratory diagnosis is important in such situations and when disease control action is desired. PCR tests can provide accurate lab diagnosis within 2 days. Two variations of a simple DNA extraction protocol using sodium hydroxide (NaOH) were compared with that of the proprietary protocol of a commercially available kit (CK) for nested PCR to detect the pathogen in oak sapwood. High frequencies of pathogen detection (98 to 100% of 48 branch segments assayed) were found for northern pin oak using the two NaOH-based and the CK methods. Detection rates were similar but lower for bur oak (ranged from 58 to 79%) and white oak (ranged from 54 to 71%) regardless of DNA extraction method. Using our alternative DNA extraction protocols may reduce total time and cost of B.

Original languageEnglish (US)
Pages (from-to)132-139
Number of pages8
JournalPlant Health Progress
Volume23
Issue number2
DOIs
StatePublished - 2022

Bibliographical note

Funding Information:
Funding: This work is supported by the Minnesota Environment and Natural Resource Trust Fund as recommended by the Legislative Citizen Commission on Minnesota’s Resources, through the Minnesota Invasive Terrestrial Plants and Pests Center, and by the United States Department of Agriculture–National Institute of Food and Agriculture (Hatch project 1006789).

Publisher Copyright:
© 2022. Plant Health Progress.All Rights Reserved.

Keywords

  • Bretziella fagacearum
  • Dna extraction
  • Oak wilt fungus
  • Quercus

Fingerprint

Dive into the research topics of 'Use of Sodium Hydroxide DNA Extraction Methods for Nested PCR Detection of Bretziella fagacearum in the Sapwood of Oak Species in Minnesota'. Together they form a unique fingerprint.

Cite this