Use of slaughterhouses as sentinel points for genomic surveillance of foot-and-mouth disease virus in southern vietnam

Umanga Gunasekara, Miranda R. Bertram, Do H. Dung, Bui H. Hoang, Nguyen T. Phuong, Vo V. Hung, Nguyen V. Long, Phan Q. Minh, Le T. Vu, Pham V. Dong, Andres M Perez, Kimberly Vanderwaal, Jonathan Arzt

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The genetic diversity of foot-and-mouth disease virus (FMDV) poses a challenge to the successful control of the disease, and it is important to identify the emergence of different strains in endemic settings. The objective of this study was to evaluate the sampling of clinically healthy livestock at slaughterhouses as a strategy for genomic FMDV surveillance. Serum samples (n = 11,875) and oropharyngeal fluid (OPF) samples (n = 5045) were collected from clinically healthy cattle and buffalo on farms in eight provinces in southern and northern Vietnam (2015–2019) to characterize viral diversity. Outbreak sequences were collected between 2009 and 2019. In two slaughterhouses in southern Vietnam, 1200 serum and OPF samples were collected from clinically healthy cattle and buffalo (2017 to 2019) as a pilot study on the use of slaughterhouses as sentinel points in surveillance. FMDV VP1 sequences were analyzed using discriminant principal component analysis and timescaled phylodynamic trees. Six of seven serotype-O and-A clusters circulating in southern Vietnam between 2017–2019 were detected at least once in slaughterhouses, sometimes pre-dating outbreak sequences associated with the same cluster by 4–6 months. Routine sampling at slaughterhouses may provide a timely and efficient strategy for genomic surveillance to identify circulating and emerging FMDV strains.

Original languageEnglish (US)
Article number2203
JournalViruses
Volume13
Issue number11
DOIs
StatePublished - Nov 2021

Bibliographical note

Funding Information:
Funding: This research was funded in part by the United States Department of Agriculture, Agricultural Research Service (ARS-CRIS Project 1940-32000-061-00D). Additional funding was provided by the Cooperative Biological Engagement Program of the U.S. Department of Defense, Defense Threat Reduction Agency (CBEP/DTRA/DOD).

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Disease control
  • Genetic diversity
  • Molecular epidemiology
  • Phylogenetics
  • Sentinels
  • Subclinical infection
  • Surveillance

PubMed: MeSH publication types

  • Journal Article
  • Research Support, U.S. Gov't, Non-P.H.S.

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