Use of real-time PCR to detect canine parvovirus in feces of free-ranging wolves

L. David Mech, Emily S. Almberg, Douglas Smith, Sagar Goyal, Randall S. Singer

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Using real-time PCR, we tested 15 wolf (Canis lupus) feces from the Superior National Forest (SNF), Minnesota, USA, and 191 from Yellowstone National Park (YNP), USA, collected during summer and 13 during winter for canine parvovirus (CPV)-2 DNA. We also tested 20 dog feces for CPV-2 DNA. The PCR assay was 100%sensitive and specific with a minimum detection threshold of 104 50% tissue culture infective dose. Virus was detected in two winter specimens but none of the summer specimens. We suggest applying the technique more broadly especially with winter feces.

Original languageEnglish (US)
Pages (from-to)473-476
Number of pages4
JournalJournal of wildlife diseases
Volume48
Issue number2
DOIs
StatePublished - 2012

Keywords

  • Canine parvovirus
  • Canis lupus
  • Feces
  • Real-time PCR
  • Wolf

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