Use of Petunia inflata as a model for the study of solanaceous type self-incompatibility

Peter E. Dowd, Andrew G. McCubbin, Xi Wang, Joseph A. Verica, Tatsuya Tsukamoto, Toshio Ando, Teh Hui Kao

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Petunia inflata has been used as a model to study solanaceous type self-incompatibility (SI), an RNase-mediated self/non-self recognition mechanism. Pistil proteins that co-segregate with S-alleles (termed S-proteins or S-RNases) were identified, and their role in SI was demonstrated by gain-of-function and loss-of-function experiments. Several lines of evidence strongly suggest that the S-locus contains another gene, termed the pollen S-gene, whose allelic products presumably interact with S-RNases to trigger the SI response. Thus, ultimate understanding of the molecular/biochemical basis of SI requires the identification of the pollen S-gene. A number of approaches that have been used to accomplish this objective are discussed. Differential display and subtractive hybridization have yielded the most promising results: cDNAs corresponding to 13 pollen-expressed genes that lie within 1 cM of the S-RNase gene have been identified. They will be used as markers to identify large S-linked genomic fragments which will then be examined for the presence of the pollen S-gene by plant transformation. (C) 2000 Annals of Botany Company.

Original languageEnglish (US)
Pages (from-to)87-93
Number of pages7
JournalAnnals of Botany
Issue numberSUPPL. A
StatePublished - Jan 1 2000


  • Petunia inflata
  • Pollen-pistil interactions
  • S-R Nases
  • S-linked cDNA markers
  • Self-incompatibility

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    Dowd, P. E., McCubbin, A. G., Wang, X., Verica, J. A., Tsukamoto, T., Ando, T., & Kao, T. H. (2000). Use of Petunia inflata as a model for the study of solanaceous type self-incompatibility. Annals of Botany, 85(SUPPL. A), 87-93.