Use of DNA marker to select well-adapted Phaseolus-symbionts strains under acid conditions and high temperature

Abdelaal Shamseldin

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Soil acidity and high temperature contribute to the failure of nodulation in the common bean. It is therefore urgent to select strains with a high competitive ability under these stress conditions. Two Egyptian Rhizobium etli strains, EBRI 2 and EBRI 26, were examined against Rhizobium tropici CIAT 899G labeled with the gus (β-glucuronidase) reporter gene. EBRI 2 and EBRI 26 were less competitive than CIAT 899G under acid conditions with both the Egyptian cultivar Giza 3 and the Colombian cultivar Rab 39. However, EBRI 2 and EBRI 26 gave higher nodule occupancy (78% and 62.5, respectively) than the nodule occupancy (18.5% and 35%) obtained by CIAT 899G at 35°C with cultivar Giza 3. Soil acidity (pH 5.8) was less detrimental to the nodule occupancy of EBRI 2 than EBRI 26 when they tested in competition with CIAT 899G.

Original languageEnglish (US)
Pages (from-to)37-44
Number of pages8
JournalBiotechnology Letters
Volume29
Issue number1
DOIs
StatePublished - Jan 2007
Externally publishedYes

Keywords

  • DNA marker
  • Phaseolus
  • Rhizobium competition
  • Soil acidity

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