TY - JOUR
T1 - Use of adoptive transfer of T-cell-antigen-receptor-transgenic T cells for the study of T-cell activation in vivo
AU - Pape, Kathryn A.
AU - Kearney, Elizabeth R.
AU - Khoruts, Alexander
AU - Mondino, Anna
AU - Merica, Rebecca
AU - Chen, Zong Ming
AU - Ingulli, Elizabeth
AU - White, Jennifer
AU - Johnson, Julia G.
AU - Jenkins, Marc K.
PY - 1997
Y1 - 1997
N2 - Adoptive transfer of TCR-transgenic T cells uniformly expressing an identifiable TCR of known peptide/MHC specificity can be used to monitor the in vivo behavior of antigen-specific T cells. We have used this system to show that naive T cells are initially activated within the T-cell zones of secondary lymphoid tissue to proliferate in a B7-dependent manner. If adjuvants or inflammatory cytokines are present during this period, enhanced numbers of T cells accumulate, migrate into B-cell-rich follicles, and acquire the capacity to produce IFN-γ and help B cells produce IgG2a. If inflammation is absent, most of the initially activated antigen-specific T cells disappear without entering the follicles, and the survivors are poor producers of IL-2 and IFN-γ. Our results indicate that inflammatory mediators play a key role in regulating the anatomic location, clonal expansion, survival and lymphokine production potential of antigen-stimulated T cells in vivo.
AB - Adoptive transfer of TCR-transgenic T cells uniformly expressing an identifiable TCR of known peptide/MHC specificity can be used to monitor the in vivo behavior of antigen-specific T cells. We have used this system to show that naive T cells are initially activated within the T-cell zones of secondary lymphoid tissue to proliferate in a B7-dependent manner. If adjuvants or inflammatory cytokines are present during this period, enhanced numbers of T cells accumulate, migrate into B-cell-rich follicles, and acquire the capacity to produce IFN-γ and help B cells produce IgG2a. If inflammation is absent, most of the initially activated antigen-specific T cells disappear without entering the follicles, and the survivors are poor producers of IL-2 and IFN-γ. Our results indicate that inflammatory mediators play a key role in regulating the anatomic location, clonal expansion, survival and lymphokine production potential of antigen-stimulated T cells in vivo.
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U2 - 10.1111/j.1600-065X.1997.tb00959.x
DO - 10.1111/j.1600-065X.1997.tb00959.x
M3 - Review article
C2 - 9176700
AN - SCOPUS:16944365812
SN - 0105-2896
VL - 156
SP - 67
EP - 78
JO - Immunological Reviews
JF - Immunological Reviews
ER -