Use of a single, triplicate arbitrarily primed-PCR procedure for molecular fingerprinting of lactic acid bacteria

Sean M. Cusick, Daniel J. O'Sullivan

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Arbitrarily primed (AP)-PCR can be used to generate characteristic DNA fingerprint patterns. However, small changes in reaction conditions can cause band irreproducibility. In this study, a single methodology encompassing triplicate reactions, which were intentionally exposed to three different annealing temperatures, enabled bands that were reproducibly generated to be recognized. A single triplicate AP-PCR (TAP-PCR) procedure, using an 18-mer primer, was developed and used to fingerprint representative isolates from the major genera of lactic acid bacteria and Bifidobacterium to the strain level.

Original languageEnglish (US)
Pages (from-to)2227-2231
Number of pages5
JournalApplied and environmental microbiology
Volume66
Issue number5
DOIs
StatePublished - May 2000

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