Urinary microbiome associated with chronic allograft dysfunction in kidney transplant recipients

Jennifer F. Wu, Amutha Muthusamy, Gabriel A. Al-Ghalith, Dan Knights, Bin Guo, Baolin Wu, Rory P Remmel, David P. Schladt, Maria Luisa Alegre, William S Oetting, Pamala A Jacobson, Ajay K. Israni

Research output: Contribution to journalArticle

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Abstract

Background: We performed a study to identify differences in the urinary microbiome associated with chronic allograft dysfunction (CAD) and compared the urinary microbiome of male and female transplant recipients with CAD. Methods: This case-control study enrolled 67 patients within the Deterioration of Kidney Allograft Function (DeKAF) Genomics cohort at two transplant centers. CAD was defined as a greater than 25% rise in serum creatinine relative to a 3 month post-transplant baseline. Urine samples from patients with and without CAD were analyzed using 16S V4 bacterial ribosomal DNA sequences. Results: Corynebacterium was more prevalent in female and male patients with CAD compared to non-CAD female patients (P = 0.0005). A total 21 distinct Operational Taxonomic Unit (OTUs) were identified as significantly different when comparing CAD and non-CAD patients using Kruskal-Wallis (P < 0.01). A subset analysis of female patients with CAD compared to non-CAD females identified similar differentially abundant OTUs, including the genera Corynebacterium and Staphylococcus (Kruskal-Wallis; P = 0.01; P = 0.004, respectively). Male CAD vs female CAD analysis showed greater abundance of phylum Proteobacteria in males. Conclusion: There were differences in the urinary microbiome when comparing female and male CAD patients with their female non-CAD counterparts and these differences persisted in the subset analysis limited to female patients only.

Original languageEnglish (US)
Article numbere13436
JournalClinical Transplantation
Volume32
Issue number12
DOIs
StatePublished - Dec 1 2018

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Microbiota
Allografts
Kidney
Corynebacterium
Transplant Recipients
Transplants
Proteobacteria
Bacterial DNA
Genomics
Ribosomal DNA
Staphylococcus
Case-Control Studies

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural

Cite this

Urinary microbiome associated with chronic allograft dysfunction in kidney transplant recipients. / Wu, Jennifer F.; Muthusamy, Amutha; Al-Ghalith, Gabriel A.; Knights, Dan; Guo, Bin; Wu, Baolin; Remmel, Rory P; Schladt, David P.; Alegre, Maria Luisa; Oetting, William S; Jacobson, Pamala A; Israni, Ajay K.

In: Clinical Transplantation, Vol. 32, No. 12, e13436, 01.12.2018.

Research output: Contribution to journalArticle

Wu, Jennifer F. ; Muthusamy, Amutha ; Al-Ghalith, Gabriel A. ; Knights, Dan ; Guo, Bin ; Wu, Baolin ; Remmel, Rory P ; Schladt, David P. ; Alegre, Maria Luisa ; Oetting, William S ; Jacobson, Pamala A ; Israni, Ajay K. / Urinary microbiome associated with chronic allograft dysfunction in kidney transplant recipients. In: Clinical Transplantation. 2018 ; Vol. 32, No. 12.
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abstract = "Background: We performed a study to identify differences in the urinary microbiome associated with chronic allograft dysfunction (CAD) and compared the urinary microbiome of male and female transplant recipients with CAD. Methods: This case-control study enrolled 67 patients within the Deterioration of Kidney Allograft Function (DeKAF) Genomics cohort at two transplant centers. CAD was defined as a greater than 25{\%} rise in serum creatinine relative to a 3 month post-transplant baseline. Urine samples from patients with and without CAD were analyzed using 16S V4 bacterial ribosomal DNA sequences. Results: Corynebacterium was more prevalent in female and male patients with CAD compared to non-CAD female patients (P = 0.0005). A total 21 distinct Operational Taxonomic Unit (OTUs) were identified as significantly different when comparing CAD and non-CAD patients using Kruskal-Wallis (P < 0.01). A subset analysis of female patients with CAD compared to non-CAD females identified similar differentially abundant OTUs, including the genera Corynebacterium and Staphylococcus (Kruskal-Wallis; P = 0.01; P = 0.004, respectively). Male CAD vs female CAD analysis showed greater abundance of phylum Proteobacteria in males. Conclusion: There were differences in the urinary microbiome when comparing female and male CAD patients with their female non-CAD counterparts and these differences persisted in the subset analysis limited to female patients only.",
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AB - Background: We performed a study to identify differences in the urinary microbiome associated with chronic allograft dysfunction (CAD) and compared the urinary microbiome of male and female transplant recipients with CAD. Methods: This case-control study enrolled 67 patients within the Deterioration of Kidney Allograft Function (DeKAF) Genomics cohort at two transplant centers. CAD was defined as a greater than 25% rise in serum creatinine relative to a 3 month post-transplant baseline. Urine samples from patients with and without CAD were analyzed using 16S V4 bacterial ribosomal DNA sequences. Results: Corynebacterium was more prevalent in female and male patients with CAD compared to non-CAD female patients (P = 0.0005). A total 21 distinct Operational Taxonomic Unit (OTUs) were identified as significantly different when comparing CAD and non-CAD patients using Kruskal-Wallis (P < 0.01). A subset analysis of female patients with CAD compared to non-CAD females identified similar differentially abundant OTUs, including the genera Corynebacterium and Staphylococcus (Kruskal-Wallis; P = 0.01; P = 0.004, respectively). Male CAD vs female CAD analysis showed greater abundance of phylum Proteobacteria in males. Conclusion: There were differences in the urinary microbiome when comparing female and male CAD patients with their female non-CAD counterparts and these differences persisted in the subset analysis limited to female patients only.

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