Upstream stimulating factor-1 mediates the E-box-dependent transcriptional repression of the plasminogen activator inhibitor-1 gene in human mast cells

Zhongcai Ma; Bong Sook Jhun; Chad K. Oh

doi:10.1016/j.febslet.2007.08.034

Upstream stimulating factor-1 mediates the E-box-dependent transcriptional repression of the plasminogen activator inhibitor-1 gene in human mast cells

Zhongcai Ma
, Bong Sook Jhun
, Chad K. Oh

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Plasminogen activator inhibitor (PAI)-1 promotes development of asthma. PAI-1 mRNA and protein are markedly induced in activated mast cells (MCs), a major effector cell type in asthma. However, regulatory mechanisms of PAI-1 transcription in MCs are unknown. We present first evidence that PAI-1 is transcriptionally regulated in human MCs (hMCs). In addition to three enhancer regions, we demonstrated that the E-box at -566 bp to -561 bp is the negative regulatory element, and the specific and constitutive binding of the upstream stimulating factor-1 to this E-box is the key mechanism of the negative regulation of PAI-1 expression in hMCs.

Original language	English (US)
Pages (from-to)	4485-4490
Number of pages	6
Journal	FEBS Letters
Volume	581
Issue number	23
DOIs	https://doi.org/10.1016/j.febslet.2007.08.034
State	Published - Sep 18 2007
Externally published	Yes

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

E-box
Human mast cells (hMCs)
Plasminogen activator inhibitor-1 (PAI-1)
Transcription
Upstream stimulating factor-1 (USF-1)

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10.1016/j.febslet.2007.08.034

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title = "Upstream stimulating factor-1 mediates the E-box-dependent transcriptional repression of the plasminogen activator inhibitor-1 gene in human mast cells",

abstract = "Plasminogen activator inhibitor (PAI)-1 promotes development of asthma. PAI-1 mRNA and protein are markedly induced in activated mast cells (MCs), a major effector cell type in asthma. However, regulatory mechanisms of PAI-1 transcription in MCs are unknown. We present first evidence that PAI-1 is transcriptionally regulated in human MCs (hMCs). In addition to three enhancer regions, we demonstrated that the E-box at -566 bp to -561 bp is the negative regulatory element, and the specific and constitutive binding of the upstream stimulating factor-1 to this E-box is the key mechanism of the negative regulation of PAI-1 expression in hMCs.",

keywords = "E-box, Human mast cells (hMCs), Plasminogen activator inhibitor-1 (PAI-1), Transcription, Upstream stimulating factor-1 (USF-1)",

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year = "2007",

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TY - JOUR

T1 - Upstream stimulating factor-1 mediates the E-box-dependent transcriptional repression of the plasminogen activator inhibitor-1 gene in human mast cells

AU - Ma, Zhongcai

AU - Jhun, Bong Sook

AU - Oh, Chad K.

PY - 2007/9/18

Y1 - 2007/9/18

N2 - Plasminogen activator inhibitor (PAI)-1 promotes development of asthma. PAI-1 mRNA and protein are markedly induced in activated mast cells (MCs), a major effector cell type in asthma. However, regulatory mechanisms of PAI-1 transcription in MCs are unknown. We present first evidence that PAI-1 is transcriptionally regulated in human MCs (hMCs). In addition to three enhancer regions, we demonstrated that the E-box at -566 bp to -561 bp is the negative regulatory element, and the specific and constitutive binding of the upstream stimulating factor-1 to this E-box is the key mechanism of the negative regulation of PAI-1 expression in hMCs.

AB - Plasminogen activator inhibitor (PAI)-1 promotes development of asthma. PAI-1 mRNA and protein are markedly induced in activated mast cells (MCs), a major effector cell type in asthma. However, regulatory mechanisms of PAI-1 transcription in MCs are unknown. We present first evidence that PAI-1 is transcriptionally regulated in human MCs (hMCs). In addition to three enhancer regions, we demonstrated that the E-box at -566 bp to -561 bp is the negative regulatory element, and the specific and constitutive binding of the upstream stimulating factor-1 to this E-box is the key mechanism of the negative regulation of PAI-1 expression in hMCs.

KW - E-box

KW - Human mast cells (hMCs)

KW - Plasminogen activator inhibitor-1 (PAI-1)

KW - Transcription

KW - Upstream stimulating factor-1 (USF-1)

UR - https://www.scopus.com/pages/publications/34548482979

UR - https://www.scopus.com/pages/publications/34548482979#tab=citedBy

U2 - 10.1016/j.febslet.2007.08.034

DO - 10.1016/j.febslet.2007.08.034

M3 - Article

C2 - 17765897

AN - SCOPUS:34548482979

SN - 0014-5793

VL - 581

SP - 4485

EP - 4490

JO - FEBS Letters

JF - FEBS Letters

IS - 23

ER -

Upstream stimulating factor-1 mediates the E-box-dependent transcriptional repression of the plasminogen activator inhibitor-1 gene in human mast cells

Abstract

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Keywords

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