Upstream stimulating factor-1 mediates the E-box-dependent transcriptional repression of the plasminogen activator inhibitor-1 gene in human mast cells

Zhongcai Ma, Bong Sook Jhun, Chad K. Oh

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Plasminogen activator inhibitor (PAI)-1 promotes development of asthma. PAI-1 mRNA and protein are markedly induced in activated mast cells (MCs), a major effector cell type in asthma. However, regulatory mechanisms of PAI-1 transcription in MCs are unknown. We present first evidence that PAI-1 is transcriptionally regulated in human MCs (hMCs). In addition to three enhancer regions, we demonstrated that the E-box at -566 bp to -561 bp is the negative regulatory element, and the specific and constitutive binding of the upstream stimulating factor-1 to this E-box is the key mechanism of the negative regulation of PAI-1 expression in hMCs.

Original languageEnglish (US)
Pages (from-to)4485-4490
Number of pages6
JournalFEBS Letters
Volume581
Issue number23
DOIs
StatePublished - Sep 18 2007
Externally publishedYes

Keywords

  • E-box
  • Human mast cells (hMCs)
  • Plasminogen activator inhibitor-1 (PAI-1)
  • Transcription
  • Upstream stimulating factor-1 (USF-1)

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