Upregulation of Polo-like kinase 2 gene expression by GATA-1 acetylation in human osteosarcoma MG-63 cells

Tao Shen, Yan Li, Liqing Yang, Xiaojun Xu, Feng Liang, Shuang Liang, Gen Ba, Feng Xue, Qin Fu

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Polo-like kinase 2 (Plk2) is a member of the serine/threonine protein kinase family involved in cell-cycle regulation and cellular response to stresses. It is of great interest to investigate the molecular mechanisms that control the expression of Plk2. Here, using real-time PCR and Western blot assays, we show that trichostatin A (TSA), a histone deacetylase inhibitor, upregulated Plk2 mRNA and protein expression in the human osteosarcoma MG-63 cell line. Luciferase activity analysis of the truncated Plk2 promoter indicated that the region from -1220 to -830 of the Plk2 promoter was sensitive to TSA. Moreover, using the electrophoresis mobility shift assay and chromatin immunoprecipitation assay, we identified two GATA-1 responsive elements at positions -1051 and -949, to which GATA-1 binding was enhanced by TSA under in vitro and in vivo conditions. Immunoprecipitation and Western blot showed that the levels of acetylated GATA-1 were increased with TSA in MG-63 cells, consistent with their binding affinities to the GATA-1 responsive elements. In summary, these data demonstrate that acetylation plays a crucial role in Plk2 expression and acetylation of GATA-1 by TSA treatment may upregulate their DNA-binding affinities, resulting in the activation of Plk2 promoter. These results may contribute to the understanding of the molecular mechanism of Plk2 regulation.

Original languageEnglish (US)
Pages (from-to)423-429
Number of pages7
JournalInternational Journal of Biochemistry and Cell Biology
Volume44
Issue number2
DOIs
StatePublished - Feb 2012

Bibliographical note

Funding Information:
We thank Dr. Hueng-Sik Choi and Yong Deuk Kim for generously providing Plk2 promoter constructs. This project is supported by grants from National Natural Science Foundation of China (No. 81070688 and No. 30800415 ).

Keywords

  • Acetylation
  • Expression regulation
  • GATA-1
  • Plk2
  • TSA

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