Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma

Hao Bo, Zhaojian Gong, Wenling Zhang, Xiayu Li, Yong Zeng, Qianjin Liao, Pan Chen, Lei Shi, Yu Lian, Yizhou Jing, Ke Tang, Zheng Li, Yanhong Zhou, Ming Zhou, Bo Xiang, Xiaoling Li, Jianbo Yang, Wei Xiong, Guiyuan Li, Zhaoyang Zeng

Research output: Contribution to journalArticlepeer-review

135 Scopus citations

Abstract

Altered expression of long noncoding RNAs (lncRNAs) associated with human carcinogenesis. We performed a cDNA microarray analysis of lncRNA expression in 12 cases of nasopharyngeal carcinoma (NPC) and 4 non-tumor nasopharyngeal epitheliums. One lncRNA, actin filament associated protein 1 antisense RNA1 (AFAP1- AS1), was identified and selected for further study. AFAP1-AS1 expression was upregulated in NPC and associated with NPC metastasis and poor prognosis. In vitro experiments demonstrated that AFAP1-AS1 knockdown significantly inhibited the NPC cell migration and invasive capability. AFAP1-AS1 knockdown also increased AFAP1 protein expression. Proteomic and bioinformatics analyses suggested that AFAP1-AS1 affected the expression of several small GTPase family members and molecules in the actin cytokeratin signaling pathway. AFAP1-AS1 promoted cancer cell metastasis via regulation of actin filament integrity. AFAP1-AS1 might be a potential novel marker that can predict cancer patient prognosis and as a potential therapeutic target for NPC.

Original languageEnglish (US)
Pages (from-to)20404-20418
Number of pages15
JournalOncotarget
Volume6
Issue number24
DOIs
StatePublished - 2015

Keywords

  • AFAP1 antisense RNA1 (AFAP1-AS1)
  • Long non-coding RNA (LncRNA)
  • Metastasis
  • Nasopharyngeal carcinoma (NPC)
  • Prognosis

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