Update of Genetic Diversity of Porcine Circovirus Type 2 in Chile Evidences the Emergence of PCV2d Genotype

Naomi Ariyama, Belén Agüero, Valentina Valdés, Felipe Berrios, Sergio Bucarey, Sunil Mor, Barbara Brito, Victor Neira

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5 Scopus citations


Porcine Circovirus 2 (PCV2) can cause multiple clinical conditions known as porcine circovirus-associated diseases (PCVAD). Before the wide availability of PCV2 vaccines, PCVAD resulted in significant losses to the global swine industry. PCV2's rapid evolutionary dynamics are comparable to single-stranded RNA viruses. Thus, shifts in the dominance and distribution of different genotypes may frequently occur, resulting in the emergence and spread of varying PCV2 genotypes and recombinant strains in swine. This study aims at identifying the PCV2 genotypes currently circulating in Chile. Seven hundred thirty-eight samples were obtained from 21 swine farms between 2020 and 2021. The samples were tested using PCR for species detection and genotyping. Sequencing and phylogenetic analyses were conducted in selected samples. PCV2 was detected in 26.9% of the PCR reactions and 67% of the sampled farms. The genotypes were determined in nine farms, PCV2a in one farm, PCV2b in four, and PCV2d in five, with PCV2b and PCV2d co-circulating in one farm. The phylogenetic analysis of twelve ORF2 sequences obtained (PCV2a = 5; PCV2b = 4; PCV2d = 3) showed a PCV2a Chilean strains monophyletic cluster; closely related to Chilean viruses collected in 2012 and 2013. Of the three different PCV2b sequenced viruses, two viruses were close to the root of the PCV2b group, whereas the remaining one grouped with a South Korean virus. PCV2d sequences were closely related to Asian viruses. A previously reported PCV2a/PCV2d recombinant strain was not detected in this study. Our results suggest the emergence and potential shift to PCV2d genotype in Chilean farms.

Original languageEnglish (US)
Article number789491
JournalFrontiers in Veterinary Science
StatePublished - Dec 17 2021

Bibliographical note

Funding Information:
This study was partially funded by the Animal Virology Laboratory, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile; Programa Fondecyt N◦ 11170877 and N◦ 1211517 to VN; Programa Fondef N◦ ID19I10135 to SB and VN.

Publisher Copyright:
Copyright © 2021 Ariyama, Agüero, Valdés, Berrios, Bucarey, Mor, Brito and Neira.


  • Chile
  • PCV2
  • PCV2 genotypes
  • genetic diversity
  • genotype shift


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