Up-regulation of heme oxygenase-1 by Korean red ginseng water extract as a cytoprotective efect in human endothelial cells

Hana Yang, Seung Eun Lee, Seong I. Jeong, Cheung Seog Park, Young Ho Jin, Yong Seek Park

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Korean red ginseng (KRG) is used worldwide as a popular traditional herbal medicine. KRG has shown beneficial effects on cardiovascular diseases, such as atherosclerosis, diabetes, and hypertension. Up-regulation of a cytoprotective protein, heme oxygenase (HO)-1, is considered to augment the cellular defense against various agents that may induce cytotoxic injury. In the present study, we demonstrate that KRG water extract induces HO-1 expression in human umbilical vein endothelial cells (HUVECs) and possible involvement of the anti-oxidant transcription factor nuclear factor-eythroid 2-related factor 2 (Nrf2). KRG-induced HO-1 expression was examined by western blots, reverse transcriptase polymerase chain reaction and immunofluorescence staining. Specifc silencing of Nrf2 genes with Nrf2-siRNA in HUVECs abolished HO-1 expression. In addition, the HO inhibitor zinc protoporphyrin blunted the preventive effect of KRG on Hinf2/infOinf2/inf-induced cell death, as demonstrated by terminal transferase dUTP nick end labeling assay. Taken together, these results suggest that KRG may exert a vasculoprotective effect through Nrf2-mediated HO-1 induction in human endothelial cell by inhibition of cell death.

Original languageEnglish (US)
Pages (from-to)352-359
Number of pages8
JournalJournal of Ginseng Research
Volume35
Issue number3
DOIs
StatePublished - Sep 2011

Bibliographical note

Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.

Keywords

  • Endothelial cells
  • Heme oxygenase
  • Oxidative stress
  • Panax ginseng
  • Vascular diseases

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