Up-regulated expression of spherical body protein 2 truncated copy 11 in Babesia bovis is associated with reduced cytoadhesion to vascular endothelial cells

Gina M. Gallego-Lopez; Audrey O.T. Lau; Roberta M. O'Connor; Massaro W. Ueti; Brian M. Cooke; Jacob M. Laughery; Telmo Graça; Sally A. Madsen-Bouterse; Daiane P. Oldiges; David R. Allred; Carlos E. Suarez

doi:10.1016/j.ijpara.2018.05.015

Up-regulated expression of spherical body protein 2 truncated copy 11 in Babesia bovis is associated with reduced cytoadhesion to vascular endothelial cells

Gina M. Gallego-Lopez, Audrey O.T. Lau, Roberta M. O'Connor, Massaro W. Ueti, Brian M. Cooke, Jacob M. Laughery, Telmo Graça, Sally A. Madsen-Bouterse, Daiane P. Oldiges, David R. Allred, Carlos E. Suarez

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

The factors involved in gain or loss of virulence in Babesia bovis are unknown. Spherical body protein 2 truncated copy 11 (sbp2t11) transcripts in B. bovis were recently reported to be a marker of attenuation for B. bovis strains. Increased cytoadhesion of B. bovis-infected red blood cells (iRBC) to vascular endothelial cells is associated with severe disease outcomes and an indicator of parasite virulence. Here, we created a stable B. bovis transfected line over-expressing sbp2t11 to determine whether up-regulation of sbp2t11 is associated with changes in cytoadhesion. This line was designated sbp2t11up and five B. bovis clonal lines were derived from the sbp2t11up line by limiting dilution for characterisation. We compared the ability of iRBCs from the sbp2t11up line and its five derivative clonal lines to adhere to bovine brain endothelial cells, using an in vitro cytoadhesion assay. The same lines were selected for in vitro cytoadhesion and the levels of sbp2t11 transcripts in each selected line were quantified. Our results demonstrate that up-regulation of sbp2t11 is accompanied by a statistically significant reduction in cytoadhesion. Confirmed up-regulation of sbp2t11 in B. bovis concomitant with the reduction of iRBC in vitro cytoadhesion to bovine brain endothelial cell is consistent with our previous finding that up-regulation of sbp2t11 is an attenuation marker in B. bovis and suggests the involvement of sbp2t11 transcription in B. bovis virulence.

Original language	English (US)
Pages (from-to)	127-137
Number of pages	11
Journal	International Journal for Parasitology
Volume	49
Issue number	2
DOIs	https://doi.org/10.1016/j.ijpara.2018.05.015
State	Published - Feb 2019
Externally published	Yes

Bibliographical note

Funding Information:
We are extremely grateful for the technical assistance from Jessica Ujcizo, Debra Alperin, Hanen Baggar, Edith Orozco, Paul Lacy, Monica Pedroni and Linda Hamburg. The design of the study, data collection and analysis, interpretation of data, and writing were funded by Washington State University (USA), College of Veterinary Medicine , intramural grant (AOTL), the United States Department of Agriculture-Agriculture Research Service Current Research Information System Project No. 2090-32000-039-00D, and the International Development Research Centre (IDRC) (Canada) project 108525-001. Gallego-Lopez was supported primarily by a Francisco Jose de Caldas Scholarship from Colciencias-Colombia.

Publisher Copyright:
© 2018

Keywords

BBEC
Babesia bovis
Cytoadhesion
Spherical body protein
Stable transfection

Publisher link

10.1016/j.ijpara.2018.05.015

Find It

Find It at U of M Libraries

Cite this

Gallego-Lopez, G. M., Lau, A. O. T., O'Connor, R. M., Ueti, M. W., Cooke, B. M., Laughery, J. M., Graça, T., Madsen-Bouterse, S. A., Oldiges, D. P., Allred, D. R., & Suarez, C. E. (2019). Up-regulated expression of spherical body protein 2 truncated copy 11 in Babesia bovis is associated with reduced cytoadhesion to vascular endothelial cells. International Journal for Parasitology, 49(2), 127-137. https://doi.org/10.1016/j.ijpara.2018.05.015

Up-regulated expression of spherical body protein 2 truncated copy 11 in Babesia bovis is associated with reduced cytoadhesion to vascular endothelial cells. / Gallego-Lopez, Gina M.; Lau, Audrey O.T.; O'Connor, Roberta M. et al.
In: International Journal for Parasitology, Vol. 49, No. 2, 02.2019, p. 127-137.

Research output: Contribution to journal › Article › peer-review

Gallego-Lopez, GM, Lau, AOT, O'Connor, RM, Ueti, MW, Cooke, BM, Laughery, JM, Graça, T, Madsen-Bouterse, SA, Oldiges, DP, Allred, DR & Suarez, CE 2019, 'Up-regulated expression of spherical body protein 2 truncated copy 11 in Babesia bovis is associated with reduced cytoadhesion to vascular endothelial cells', International Journal for Parasitology, vol. 49, no. 2, pp. 127-137. https://doi.org/10.1016/j.ijpara.2018.05.015

@article{0c9a8606402c49bd891665d02579f85b,

title = "Up-regulated expression of spherical body protein 2 truncated copy 11 in Babesia bovis is associated with reduced cytoadhesion to vascular endothelial cells",

abstract = "The factors involved in gain or loss of virulence in Babesia bovis are unknown. Spherical body protein 2 truncated copy 11 (sbp2t11) transcripts in B. bovis were recently reported to be a marker of attenuation for B. bovis strains. Increased cytoadhesion of B. bovis-infected red blood cells (iRBC) to vascular endothelial cells is associated with severe disease outcomes and an indicator of parasite virulence. Here, we created a stable B. bovis transfected line over-expressing sbp2t11 to determine whether up-regulation of sbp2t11 is associated with changes in cytoadhesion. This line was designated sbp2t11up and five B. bovis clonal lines were derived from the sbp2t11up line by limiting dilution for characterisation. We compared the ability of iRBCs from the sbp2t11up line and its five derivative clonal lines to adhere to bovine brain endothelial cells, using an in vitro cytoadhesion assay. The same lines were selected for in vitro cytoadhesion and the levels of sbp2t11 transcripts in each selected line were quantified. Our results demonstrate that up-regulation of sbp2t11 is accompanied by a statistically significant reduction in cytoadhesion. Confirmed up-regulation of sbp2t11 in B. bovis concomitant with the reduction of iRBC in vitro cytoadhesion to bovine brain endothelial cell is consistent with our previous finding that up-regulation of sbp2t11 is an attenuation marker in B. bovis and suggests the involvement of sbp2t11 transcription in B. bovis virulence.",

keywords = "BBEC, Babesia bovis, Cytoadhesion, Spherical body protein, Stable transfection",

author = "Gallego-Lopez, {Gina M.} and Lau, {Audrey O.T.} and O'Connor, {Roberta M.} and Ueti, {Massaro W.} and Cooke, {Brian M.} and Laughery, {Jacob M.} and Telmo Gra{\c c}a and Madsen-Bouterse, {Sally A.} and Oldiges, {Daiane P.} and Allred, {David R.} and Suarez, {Carlos E.}",

note = "Funding Information: We are extremely grateful for the technical assistance from Jessica Ujcizo, Debra Alperin, Hanen Baggar, Edith Orozco, Paul Lacy, Monica Pedroni and Linda Hamburg. The design of the study, data collection and analysis, interpretation of data, and writing were funded by Washington State University (USA), College of Veterinary Medicine , intramural grant (AOTL), the United States Department of Agriculture-Agriculture Research Service Current Research Information System Project No. 2090-32000-039-00D, and the International Development Research Centre (IDRC) (Canada) project 108525-001. Gallego-Lopez was supported primarily by a Francisco Jose de Caldas Scholarship from Colciencias-Colombia. Publisher Copyright: {\textcopyright} 2018",

year = "2019",

month = feb,

doi = "10.1016/j.ijpara.2018.05.015",

language = "English (US)",

volume = "49",

pages = "127--137",

journal = "International Journal for Parasitology",

issn = "0020-7519",

publisher = "Elsevier Ltd",

number = "2",

}

TY - JOUR

T1 - Up-regulated expression of spherical body protein 2 truncated copy 11 in Babesia bovis is associated with reduced cytoadhesion to vascular endothelial cells

AU - Gallego-Lopez, Gina M.

AU - Lau, Audrey O.T.

AU - O'Connor, Roberta M.

AU - Ueti, Massaro W.

AU - Cooke, Brian M.

AU - Laughery, Jacob M.

AU - Graça, Telmo

AU - Madsen-Bouterse, Sally A.

AU - Oldiges, Daiane P.

AU - Allred, David R.

AU - Suarez, Carlos E.

N1 - Funding Information: We are extremely grateful for the technical assistance from Jessica Ujcizo, Debra Alperin, Hanen Baggar, Edith Orozco, Paul Lacy, Monica Pedroni and Linda Hamburg. The design of the study, data collection and analysis, interpretation of data, and writing were funded by Washington State University (USA), College of Veterinary Medicine , intramural grant (AOTL), the United States Department of Agriculture-Agriculture Research Service Current Research Information System Project No. 2090-32000-039-00D, and the International Development Research Centre (IDRC) (Canada) project 108525-001. Gallego-Lopez was supported primarily by a Francisco Jose de Caldas Scholarship from Colciencias-Colombia. Publisher Copyright: © 2018

PY - 2019/2

Y1 - 2019/2

N2 - The factors involved in gain or loss of virulence in Babesia bovis are unknown. Spherical body protein 2 truncated copy 11 (sbp2t11) transcripts in B. bovis were recently reported to be a marker of attenuation for B. bovis strains. Increased cytoadhesion of B. bovis-infected red blood cells (iRBC) to vascular endothelial cells is associated with severe disease outcomes and an indicator of parasite virulence. Here, we created a stable B. bovis transfected line over-expressing sbp2t11 to determine whether up-regulation of sbp2t11 is associated with changes in cytoadhesion. This line was designated sbp2t11up and five B. bovis clonal lines were derived from the sbp2t11up line by limiting dilution for characterisation. We compared the ability of iRBCs from the sbp2t11up line and its five derivative clonal lines to adhere to bovine brain endothelial cells, using an in vitro cytoadhesion assay. The same lines were selected for in vitro cytoadhesion and the levels of sbp2t11 transcripts in each selected line were quantified. Our results demonstrate that up-regulation of sbp2t11 is accompanied by a statistically significant reduction in cytoadhesion. Confirmed up-regulation of sbp2t11 in B. bovis concomitant with the reduction of iRBC in vitro cytoadhesion to bovine brain endothelial cell is consistent with our previous finding that up-regulation of sbp2t11 is an attenuation marker in B. bovis and suggests the involvement of sbp2t11 transcription in B. bovis virulence.

AB - The factors involved in gain or loss of virulence in Babesia bovis are unknown. Spherical body protein 2 truncated copy 11 (sbp2t11) transcripts in B. bovis were recently reported to be a marker of attenuation for B. bovis strains. Increased cytoadhesion of B. bovis-infected red blood cells (iRBC) to vascular endothelial cells is associated with severe disease outcomes and an indicator of parasite virulence. Here, we created a stable B. bovis transfected line over-expressing sbp2t11 to determine whether up-regulation of sbp2t11 is associated with changes in cytoadhesion. This line was designated sbp2t11up and five B. bovis clonal lines were derived from the sbp2t11up line by limiting dilution for characterisation. We compared the ability of iRBCs from the sbp2t11up line and its five derivative clonal lines to adhere to bovine brain endothelial cells, using an in vitro cytoadhesion assay. The same lines were selected for in vitro cytoadhesion and the levels of sbp2t11 transcripts in each selected line were quantified. Our results demonstrate that up-regulation of sbp2t11 is accompanied by a statistically significant reduction in cytoadhesion. Confirmed up-regulation of sbp2t11 in B. bovis concomitant with the reduction of iRBC in vitro cytoadhesion to bovine brain endothelial cell is consistent with our previous finding that up-regulation of sbp2t11 is an attenuation marker in B. bovis and suggests the involvement of sbp2t11 transcription in B. bovis virulence.

KW - BBEC

KW - Babesia bovis

KW - Cytoadhesion

KW - Spherical body protein

KW - Stable transfection

UR - http://www.scopus.com/inward/record.url?scp=85055665389&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055665389&partnerID=8YFLogxK

U2 - 10.1016/j.ijpara.2018.05.015

DO - 10.1016/j.ijpara.2018.05.015

M3 - Article

C2 - 30367864

AN - SCOPUS:85055665389

SN - 0020-7519

VL - 49

SP - 127

EP - 137

JO - International Journal for Parasitology

JF - International Journal for Parasitology

IS - 2

ER -

Up-regulated expression of spherical body protein 2 truncated copy 11 in Babesia bovis is associated with reduced cytoadhesion to vascular endothelial cells

Abstract

Bibliographical note

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this