Uncovering potential 'herbal probiotics' in Juzen-taiho-to through the study of associated bacterial populations

Diego Montenegro; Kriti Kalpana; Christine Chrissian; Ashutosh Sharma; Anna Takaoka; Maria Iacovidou; Clifford E. Soll; Olga Aminova; Adriana Heguy; Lisa Cohen; Steven Shen; Akira Kawamura

doi:10.1016/j.bmcl.2014.12.036

Uncovering potential 'herbal probiotics' in Juzen-taiho-to through the study of associated bacterial populations

Diego Montenegro, Kriti Kalpana, Christine Chrissian, Ashutosh Sharma, Anna Takaoka, Maria Iacovidou, Clifford E. Soll, Olga Aminova, Adriana Heguy, Lisa Cohen, Steven Shen, Akira Kawamura

Institute for Health Informatics

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Juzen-taiho-to (JTT) is an immune-boosting formulation of ten medicinal herbs. It is used clinically in East Asia to boost the human immune functions. The active factors in JTT have not been clarified. But, existing evidence suggests that lipopolysaccharide (LPS)-like factors contribute to the activity. To examine this possibility, JTT was subjected to a series of analyses, including high resolution mass spectrometry, which suggested the presence of structural variants of LPS. This finding opened a possibility that JTT contains immune-boosting bacteria. As the first step to characterize the bacteria in JTT, 16S ribosomal RNA sequencing was carried out for Angelica sinensis (dried root), one of the most potent immunostimulatory herbs in JTT. The sequencing revealed a total of 519 bacteria genera in A. sinensis. The most abundant genus was Rahnella, which is widely distributed in water and plants. The abundance of Rahnella appeared to correlate with the immunostimulatory activity of A. sinensis. In conclusion, the current study provided new pieces of evidence supporting the emerging theory of bacterial contribution in immune-boosting herbs.

Original language	English (US)
Pages (from-to)	466-469
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	25
Issue number	3
DOIs	https://doi.org/10.1016/j.bmcl.2014.12.036
State	Published - Feb 1 2015

Bibliographical note

Funding Information:
This study was supported in part by NIGMS/NIH SC3 GM094070 and by PSC CUNY grant TRADB-45-509 . We also acknowledge NIMHD/NIH G12 MD007599-27 , which supports the Center for Translational and Basic Research at Hunter College. The NYULMC Genome Technology Center is partially supported by the Cancer Center Support Grant , P30CA016087 , at the Laura and Isaac Perlmutter Cancer Center. S.S. acknowledges 5R01 GM064844-07 and Howard Hughes Medical Institute-HCIA Project 4CM 09/01/2012-08/31/2016.

Publisher Copyright:
© 2014 Elsevier Ltd. All rights reserved.

Keywords

Herbal medicine
Lipopolysaccharide
Mass spectrometry
Metagenomics
Next-generation sequencing
Rhizobacteria

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10.1016/j.bmcl.2014.12.036

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Montenegro, D., Kalpana, K., Chrissian, C., Sharma, A., Takaoka, A., Iacovidou, M., Soll, C. E., Aminova, O., Heguy, A., Cohen, L., Shen, S., & Kawamura, A. (2015). Uncovering potential 'herbal probiotics' in Juzen-taiho-to through the study of associated bacterial populations. Bioorganic and Medicinal Chemistry Letters, 25(3), 466-469. https://doi.org/10.1016/j.bmcl.2014.12.036

Montenegro, D, Kalpana, K, Chrissian, C, Sharma, A, Takaoka, A, Iacovidou, M, Soll, CE, Aminova, O, Heguy, A, Cohen, L, Shen, S & Kawamura, A 2015, 'Uncovering potential 'herbal probiotics' in Juzen-taiho-to through the study of associated bacterial populations', Bioorganic and Medicinal Chemistry Letters, vol. 25, no. 3, pp. 466-469. https://doi.org/10.1016/j.bmcl.2014.12.036

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abstract = "Juzen-taiho-to (JTT) is an immune-boosting formulation of ten medicinal herbs. It is used clinically in East Asia to boost the human immune functions. The active factors in JTT have not been clarified. But, existing evidence suggests that lipopolysaccharide (LPS)-like factors contribute to the activity. To examine this possibility, JTT was subjected to a series of analyses, including high resolution mass spectrometry, which suggested the presence of structural variants of LPS. This finding opened a possibility that JTT contains immune-boosting bacteria. As the first step to characterize the bacteria in JTT, 16S ribosomal RNA sequencing was carried out for Angelica sinensis (dried root), one of the most potent immunostimulatory herbs in JTT. The sequencing revealed a total of 519 bacteria genera in A. sinensis. The most abundant genus was Rahnella, which is widely distributed in water and plants. The abundance of Rahnella appeared to correlate with the immunostimulatory activity of A. sinensis. In conclusion, the current study provided new pieces of evidence supporting the emerging theory of bacterial contribution in immune-boosting herbs.",

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author = "Diego Montenegro and Kriti Kalpana and Christine Chrissian and Ashutosh Sharma and Anna Takaoka and Maria Iacovidou and Soll, {Clifford E.} and Olga Aminova and Adriana Heguy and Lisa Cohen and Steven Shen and Akira Kawamura",

note = "Funding Information: This study was supported in part by NIGMS/NIH SC3 GM094070 and by PSC CUNY grant TRADB-45-509 . We also acknowledge NIMHD/NIH G12 MD007599-27 , which supports the Center for Translational and Basic Research at Hunter College. The NYULMC Genome Technology Center is partially supported by the Cancer Center Support Grant , P30CA016087 , at the Laura and Isaac Perlmutter Cancer Center. S.S. acknowledges 5R01 GM064844-07 and Howard Hughes Medical Institute-HCIA Project 4CM 09/01/2012-08/31/2016. Publisher Copyright: {\textcopyright} 2014 Elsevier Ltd. All rights reserved.",

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AU - Montenegro, Diego

AU - Kalpana, Kriti

AU - Chrissian, Christine

AU - Sharma, Ashutosh

AU - Takaoka, Anna

AU - Iacovidou, Maria

AU - Soll, Clifford E.

AU - Aminova, Olga

AU - Heguy, Adriana

AU - Cohen, Lisa

AU - Shen, Steven

AU - Kawamura, Akira

N1 - Funding Information: This study was supported in part by NIGMS/NIH SC3 GM094070 and by PSC CUNY grant TRADB-45-509 . We also acknowledge NIMHD/NIH G12 MD007599-27 , which supports the Center for Translational and Basic Research at Hunter College. The NYULMC Genome Technology Center is partially supported by the Cancer Center Support Grant , P30CA016087 , at the Laura and Isaac Perlmutter Cancer Center. S.S. acknowledges 5R01 GM064844-07 and Howard Hughes Medical Institute-HCIA Project 4CM 09/01/2012-08/31/2016. Publisher Copyright: © 2014 Elsevier Ltd. All rights reserved.

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N2 - Juzen-taiho-to (JTT) is an immune-boosting formulation of ten medicinal herbs. It is used clinically in East Asia to boost the human immune functions. The active factors in JTT have not been clarified. But, existing evidence suggests that lipopolysaccharide (LPS)-like factors contribute to the activity. To examine this possibility, JTT was subjected to a series of analyses, including high resolution mass spectrometry, which suggested the presence of structural variants of LPS. This finding opened a possibility that JTT contains immune-boosting bacteria. As the first step to characterize the bacteria in JTT, 16S ribosomal RNA sequencing was carried out for Angelica sinensis (dried root), one of the most potent immunostimulatory herbs in JTT. The sequencing revealed a total of 519 bacteria genera in A. sinensis. The most abundant genus was Rahnella, which is widely distributed in water and plants. The abundance of Rahnella appeared to correlate with the immunostimulatory activity of A. sinensis. In conclusion, the current study provided new pieces of evidence supporting the emerging theory of bacterial contribution in immune-boosting herbs.

AB - Juzen-taiho-to (JTT) is an immune-boosting formulation of ten medicinal herbs. It is used clinically in East Asia to boost the human immune functions. The active factors in JTT have not been clarified. But, existing evidence suggests that lipopolysaccharide (LPS)-like factors contribute to the activity. To examine this possibility, JTT was subjected to a series of analyses, including high resolution mass spectrometry, which suggested the presence of structural variants of LPS. This finding opened a possibility that JTT contains immune-boosting bacteria. As the first step to characterize the bacteria in JTT, 16S ribosomal RNA sequencing was carried out for Angelica sinensis (dried root), one of the most potent immunostimulatory herbs in JTT. The sequencing revealed a total of 519 bacteria genera in A. sinensis. The most abundant genus was Rahnella, which is widely distributed in water and plants. The abundance of Rahnella appeared to correlate with the immunostimulatory activity of A. sinensis. In conclusion, the current study provided new pieces of evidence supporting the emerging theory of bacterial contribution in immune-boosting herbs.

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KW - Metagenomics

KW - Next-generation sequencing

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ER -

Uncovering potential 'herbal probiotics' in Juzen-taiho-to through the study of associated bacterial populations

Abstract

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