Uncovering divergent evolution of α/β-hydrolases: A surprising residue substitution needed to convert Hevea brasiliensis hydroxynitrile lyase into an esterase

Keyphrases

• Hydrolases 100%
• Esterase 100%
• Residue Substitutions 100%
• Hydroxynitrile Lyase 100%
• Divergent Evolution 100%
• Hevea Brasiliensis 100%
• Esterase Activity 90%
• Glutamate 30%
• Active Sites 30%
• Catalytic Triad 30%
• Lipase 20%
• Histidine 20%
• Site Differences 20%
• P-nitrophenyl Acetate 20%
• Salicylic Acid-binding Protein 20%
• Amino Acids 10%
• Candida 10%
• Threonine 10%
• Lysine 10%
• Molecular Modeling 10%
• Catalytic Reaction 10%
• Negatively Charged 10%
• X-ray Crystallography 10%
• Hydrolysis 10%
• Side Chain 10%
• Protein Folding 10%
• Amino Acid Residues 10%
• Amino Acid Substitution 10%
• Sequence Identity 10%
• Amino Acid Sequence 10%
• Kcat 10%
• Binding Residues 10%
• Specificity Constant 10%
• Saturation mutagenesis 10%
• Ester Hydrolysis 10%
• Ser-His 10%
• Mandelonitrile 10%
• Acetone Cyanohydrin 10%
• Subtle Interaction 10%
• Hydroxynitrile 10%
• Mechanistic Studies 10%

Biochemistry, Genetics and Molecular Biology

• Hydrolase 100%
• Lyase 100%
• Esterase 100%
• Hevea Brasiliensis 100%
• Active Site 33%
• Catalytic Triad 25%
• Glutamic Acid 25%
• Amino Acids 16%
• Binding Protein 16%
• Enzymatic Hydrolysis 16%
• Enzyme 16%
• Triacylglycerol Lipase 16%
• Pancreatic Lipase 16%
• Histidine 16%
• Mutagenesis 8%
• Wild Type 8%
• X-Ray Crystallography 8%
• Peptide Sequence 8%
• Specificity Constant 8%
• Candida 8%
• Molecular Model 8%
• Turnover Number 8%
• Amino Acid Substitution 8%
• Threonine 8%
• Lysine 8%
• Protein Tertiary Structure 8%

Chemistry

• Hydroxynitrile 100%
• Amino Acid 27%
• Glutamic Acid 27%
• Salicylic Acid 18%
• Histidine 18%
• Binding Protein 18%
• Mechanistic Study 9%
• Kcat 9%
• Protein Tertiary Structure 9%
• Ser-His 9%
• Mandelonitrile 9%
• X-Ray Crystallography 9%
• Cyanohydrin 9%
• Catalytic Reaction 9%
• Lysine 9%
• Enzymatic Hydrolysis 9%
• Amino Acid Sequence 9%
• Threonine 9%
• Molecular Model 9%
• hydrolysis of esters 9%