Uncovering DELLA-independent gibberellin responses by characterizing new tomato procera mutants

Sivan Livne, Vai S. Lor, Ido Nir, Natanella Eliaz, Asaph Aharoni, Neil E. Olszewski, Yuval Eshed, David Weiss

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Gibberellin (GA) regulates plant development primarily by triggering the degradation/deactivation of the DELLA proteins. However, it remains unclear whether all GA responses are regulated by DELLAs. Tomato (Solanum lycopersicum) has a single DELLA gene named PROCERA (PRO), and its recessivepro allele exhibits constitutive GA activity but retains responsiveness to externalGA. In the loss-of-function mutant proΔGRAS, all examined GA developmental responses were considerably enhanced relative to pro and a defect in seed desiccation tolerance was uncovered. As pro, but not proΔGRAS, elongation was promoted by GA treatment, pro may retain residual DELLA activity. In agreement with homeostatic feedback regulation of the GA biosynthetic pathway, we found that GA20oxidase1 expression was suppressed in proΔGRAS and was not affected by exogenous GA3. In contrast, expression of GA2oxidase4 was not affected by the elevated GA signaling in proΔGRAS but was strongly induced by exogenous GA3. Since a similar response was found in Arabidopsis thaliana plants with impaired activity of all five DELLA genes, we suggest that homeostatic GAresponses are regulated by both DELLA-dependent and -independent pathways. Transcriptome analysis of GA-treated proΔGRAS leaves suggests that 5% of allGA-regulated genes in tomato are DELLA independent.

Original languageEnglish (US)
Pages (from-to)1579-1594
Number of pages16
JournalPlant Cell
Volume27
Issue number6
DOIs
StatePublished - Jun 1 2015

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