TY - JOUR
T1 - UNC-45A is required for neurite extension via controlling NMII activation
AU - Iizuka, Yoshie
AU - Mooneyham, Ashley
AU - Sieben, Andrew
AU - Chen, Kevin
AU - Maile, Makayla
AU - Hellweg, Raffaele
AU - Schütz, Florian
AU - Teckle, Kebebush
AU - Starr, Timothy
AU - Thayanithy, Venugopal
AU - Vogel, Rachel Isaksson
AU - Lou, Emil
AU - Lee, Michael K.
AU - Bazzaro, Martina
N1 - Publisher Copyright:
© 2017 Iizuka, Mooneyham et al.
PY - 2017/5/15
Y1 - 2017/5/15
N2 - UNC-45A is a highly conserved member of the UNC-45/CRO1/She4p family of proteins, which act as chaperones for conventional and nonconventional myosins. NMII mediates contractility and actin-based motility, which are fundamental for proper growth cone motility and neurite extension. The presence and role of UNC-45A in neuronal differentiation have been largely unknown. Here we demonstrate that UNC-45A is a novel growth cone-localized, NMII-associated component of the multiprotein complex regulating growth cone dynamics. We show that UNC-45A is dispensable for neuron survival but required for neurite elongation. Mechanistically, loss of UNC-45A results in increased levels of NMII activation. Collectively our results provide novel insights into the molecular mechanisms of neurite growth and define UNC-45A as a novel and master regulator of NMII-mediated cellular processes in neurons.
AB - UNC-45A is a highly conserved member of the UNC-45/CRO1/She4p family of proteins, which act as chaperones for conventional and nonconventional myosins. NMII mediates contractility and actin-based motility, which are fundamental for proper growth cone motility and neurite extension. The presence and role of UNC-45A in neuronal differentiation have been largely unknown. Here we demonstrate that UNC-45A is a novel growth cone-localized, NMII-associated component of the multiprotein complex regulating growth cone dynamics. We show that UNC-45A is dispensable for neuron survival but required for neurite elongation. Mechanistically, loss of UNC-45A results in increased levels of NMII activation. Collectively our results provide novel insights into the molecular mechanisms of neurite growth and define UNC-45A as a novel and master regulator of NMII-mediated cellular processes in neurons.
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U2 - 10.1091/mbc.E16-06-0381
DO - 10.1091/mbc.E16-06-0381
M3 - Article
C2 - 28356421
AN - SCOPUS:85019734711
SN - 1059-1524
VL - 28
SP - 1337
EP - 1346
JO - Molecular biology of the cell
JF - Molecular biology of the cell
IS - 10
ER -
