TY - JOUR
T1 - Umbilical Cord and Placental Blood Hematopoietic Stem Cells
T2 - Collection, Cryopreservation, and Storage
AU - Wagner, John E.
AU - Broxmeyer, Hal E.
AU - Cooper, Scott
PY - 1992
Y1 - 1992
N2 - Human umbilical cord and placental blood is a rich source of hematopoietic stem cells that can be used to reconstitute hematopoiesis after intensive myeloablative therapy. Therefore, it has become necessary to develop techniques for (i) collecting large volumes of umbilical cord aseptically, (ii) optimizing the yield of viable cells after cryopreservation and thawing, and (iii) minimizing the risk of maternal T-cell contamination. Our protocol for the collection, cryopreservation, storage, and transport of human umbilical cord and placental blood is described in detail.
AB - Human umbilical cord and placental blood is a rich source of hematopoietic stem cells that can be used to reconstitute hematopoiesis after intensive myeloablative therapy. Therefore, it has become necessary to develop techniques for (i) collecting large volumes of umbilical cord aseptically, (ii) optimizing the yield of viable cells after cryopreservation and thawing, and (iii) minimizing the risk of maternal T-cell contamination. Our protocol for the collection, cryopreservation, storage, and transport of human umbilical cord and placental blood is described in detail.
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U2 - 10.1089/scd.1.1992.1.167
DO - 10.1089/scd.1.1992.1.167
M3 - Article
C2 - 1365024
AN - SCOPUS:0026884047
SN - 1547-3287
VL - 1
SP - 167
EP - 173
JO - Stem Cells and Development
JF - Stem Cells and Development
IS - 2
ER -