Ultrastable combined atomic force and total internal fluorescence microscope

H. Gumpp; S. W. Stahl; M. Strackharn; E. M. Puchner; H. E. Gaub

doi:10.1063/1.3148224

Ultrastable combined atomic force and total internal fluorescence microscope

H. Gumpp, S. W. Stahl, M. Strackharn, E. M. Puchner, H. E. Gaub

Physics and Astronomy (Twin Cities)

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

Combining atomic force microscope (AFM) with other microscopy techniques has expanded the range of potential applications for single molecule investigations dramatically. Particularly hybrid instruments with total internal reflection fluorescence (TIRF) excitation have opened new routes in life sciences. Here we present a novel design for such a hybrid microscope, which overcomes the limitations of conventional combinations caused by their limited mechanical stability. A thorough analysis of the noise spectra and a comparison of the different designs and the different operation modes are given. With this instrument we demonstrate single molecule manipulation by AFM and simultaneous TIRF imaging.

Original language	English (US)
Article number	063704
Journal	Review of Scientific Instruments
Volume	80
Issue number	6
DOIs	https://doi.org/10.1063/1.3148224
State	Published - 2009

Bibliographical note

Funding Information:
We thank Ferdinand Kühner, Matthias Erdmann, and Jan Opfer for helpful discussions. This work was supported by the Nanosystems Initiative Munich (NIM) and the Stiftung Volkswagenwerk.

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10.1063/1.3148224

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abstract = "Combining atomic force microscope (AFM) with other microscopy techniques has expanded the range of potential applications for single molecule investigations dramatically. Particularly hybrid instruments with total internal reflection fluorescence (TIRF) excitation have opened new routes in life sciences. Here we present a novel design for such a hybrid microscope, which overcomes the limitations of conventional combinations caused by their limited mechanical stability. A thorough analysis of the noise spectra and a comparison of the different designs and the different operation modes are given. With this instrument we demonstrate single molecule manipulation by AFM and simultaneous TIRF imaging.",

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AU - Stahl, S. W.

AU - Strackharn, M.

AU - Puchner, E. M.

AU - Gaub, H. E.

N1 - Funding Information: We thank Ferdinand Kühner, Matthias Erdmann, and Jan Opfer for helpful discussions. This work was supported by the Nanosystems Initiative Munich (NIM) and the Stiftung Volkswagenwerk.

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Y1 - 2009

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AB - Combining atomic force microscope (AFM) with other microscopy techniques has expanded the range of potential applications for single molecule investigations dramatically. Particularly hybrid instruments with total internal reflection fluorescence (TIRF) excitation have opened new routes in life sciences. Here we present a novel design for such a hybrid microscope, which overcomes the limitations of conventional combinations caused by their limited mechanical stability. A thorough analysis of the noise spectra and a comparison of the different designs and the different operation modes are given. With this instrument we demonstrate single molecule manipulation by AFM and simultaneous TIRF imaging.

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Ultrastable combined atomic force and total internal fluorescence microscope

Abstract

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