UGT2B10 genotype influences nicotine glucuronidation, oxidation, and consumption

Jeannette Zinggeler Berg, Linda B von Weymarn, Elizabeth A. Thompson, Katherine M. Wickham, Natalie A. Weisensel, Dorothy K Hatsukami, Sharon E Murphy

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Tobacco exposure is routinely assessed by quantifying nicotine metabolites in plasma or urine. On average, 80% of nicotine undergoes C-oxidation to cotinine. However, interindividual variation in nicotine glucuronidation is substantial, and glucuronidation accounts for from 0% to 40% of total nicotine metabolism. We report here the effect of a polymorphism in a UDP-glucuronsyltransferase, UGT2B10, on nicotine metabolism and consumption. Methods: Nicotine, cotinine, their N-glucuronide conjugates, and total trans-3′-hydroxycotinine were quantified in the urine (n = 327) and plasma (n = 115) of smokers. Urinary nicotine N-oxide was quantified in 105 smokers. Nicotine equivalents, the sum of nicotine and all major metabolites, were calculated for each smoker. The relationship of the UGT2B10 Asp67Tyr allele to nicotine equivalents, N-glucuronidation, and C-oxidation was determined. Results: Individuals heterozygous for the Asp67Tyr allele excreted less nicotine or cotinine as their glucuronide conjugates than did wild-type, resulting in a 60% lower ratio of cotinine glucuronide to cotinine, a 50% lower ratio of nicotine glucuronide to nicotine, and increased cotinine and trans-3′-hydroxycotinine. Nicotine equivalents, a robust biomarker of nicotine intake, were lower among Asp67Tyr heterozygotes compared with individuals without this allele: 58.2 (95% confidence interval, 48.9-68.2) versus 69.2 nmol/mL (95% confidence interval, 64.3-74.5). Conclusions: Individuals heterozygous for UGT2B10 Asp67Tyr consume less nicotine than do wild-type smokers. This striking observation suggests that variations in nicotine N-glucuronidation, as reported for nicotine C-oxidation, may influence smoking behavior. Impact: UGT2B10 genotype influences nicotine metabolism and should be taken into account when characterizing the role of nicotine metabolism on smoking.

Original languageEnglish (US)
Pages (from-to)1423-1431
Number of pages9
JournalCancer Epidemiology Biomarkers and Prevention
Volume19
Issue number6
DOIs
StatePublished - Jan 1 2010

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Nicotine
Genotype
Cotinine
Alleles
Glucuronides
Smoking
Urine
Confidence Intervals
Uridine Diphosphate
Heterozygote

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UGT2B10 genotype influences nicotine glucuronidation, oxidation, and consumption. / Berg, Jeannette Zinggeler; von Weymarn, Linda B; Thompson, Elizabeth A.; Wickham, Katherine M.; Weisensel, Natalie A.; Hatsukami, Dorothy K; Murphy, Sharon E.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 19, No. 6, 01.01.2010, p. 1423-1431.

Research output: Contribution to journalArticle

Berg, Jeannette Zinggeler ; von Weymarn, Linda B ; Thompson, Elizabeth A. ; Wickham, Katherine M. ; Weisensel, Natalie A. ; Hatsukami, Dorothy K ; Murphy, Sharon E. / UGT2B10 genotype influences nicotine glucuronidation, oxidation, and consumption. In: Cancer Epidemiology Biomarkers and Prevention. 2010 ; Vol. 19, No. 6. pp. 1423-1431.
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abstract = "Background: Tobacco exposure is routinely assessed by quantifying nicotine metabolites in plasma or urine. On average, 80{\%} of nicotine undergoes C-oxidation to cotinine. However, interindividual variation in nicotine glucuronidation is substantial, and glucuronidation accounts for from 0{\%} to 40{\%} of total nicotine metabolism. We report here the effect of a polymorphism in a UDP-glucuronsyltransferase, UGT2B10, on nicotine metabolism and consumption. Methods: Nicotine, cotinine, their N-glucuronide conjugates, and total trans-3′-hydroxycotinine were quantified in the urine (n = 327) and plasma (n = 115) of smokers. Urinary nicotine N-oxide was quantified in 105 smokers. Nicotine equivalents, the sum of nicotine and all major metabolites, were calculated for each smoker. The relationship of the UGT2B10 Asp67Tyr allele to nicotine equivalents, N-glucuronidation, and C-oxidation was determined. Results: Individuals heterozygous for the Asp67Tyr allele excreted less nicotine or cotinine as their glucuronide conjugates than did wild-type, resulting in a 60{\%} lower ratio of cotinine glucuronide to cotinine, a 50{\%} lower ratio of nicotine glucuronide to nicotine, and increased cotinine and trans-3′-hydroxycotinine. Nicotine equivalents, a robust biomarker of nicotine intake, were lower among Asp67Tyr heterozygotes compared with individuals without this allele: 58.2 (95{\%} confidence interval, 48.9-68.2) versus 69.2 nmol/mL (95{\%} confidence interval, 64.3-74.5). Conclusions: Individuals heterozygous for UGT2B10 Asp67Tyr consume less nicotine than do wild-type smokers. This striking observation suggests that variations in nicotine N-glucuronidation, as reported for nicotine C-oxidation, may influence smoking behavior. Impact: UGT2B10 genotype influences nicotine metabolism and should be taken into account when characterizing the role of nicotine metabolism on smoking.",
author = "Berg, {Jeannette Zinggeler} and {von Weymarn}, {Linda B} and Thompson, {Elizabeth A.} and Wickham, {Katherine M.} and Weisensel, {Natalie A.} and Hatsukami, {Dorothy K} and Murphy, {Sharon E}",
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T1 - UGT2B10 genotype influences nicotine glucuronidation, oxidation, and consumption

AU - Berg, Jeannette Zinggeler

AU - von Weymarn, Linda B

AU - Thompson, Elizabeth A.

AU - Wickham, Katherine M.

AU - Weisensel, Natalie A.

AU - Hatsukami, Dorothy K

AU - Murphy, Sharon E

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Background: Tobacco exposure is routinely assessed by quantifying nicotine metabolites in plasma or urine. On average, 80% of nicotine undergoes C-oxidation to cotinine. However, interindividual variation in nicotine glucuronidation is substantial, and glucuronidation accounts for from 0% to 40% of total nicotine metabolism. We report here the effect of a polymorphism in a UDP-glucuronsyltransferase, UGT2B10, on nicotine metabolism and consumption. Methods: Nicotine, cotinine, their N-glucuronide conjugates, and total trans-3′-hydroxycotinine were quantified in the urine (n = 327) and plasma (n = 115) of smokers. Urinary nicotine N-oxide was quantified in 105 smokers. Nicotine equivalents, the sum of nicotine and all major metabolites, were calculated for each smoker. The relationship of the UGT2B10 Asp67Tyr allele to nicotine equivalents, N-glucuronidation, and C-oxidation was determined. Results: Individuals heterozygous for the Asp67Tyr allele excreted less nicotine or cotinine as their glucuronide conjugates than did wild-type, resulting in a 60% lower ratio of cotinine glucuronide to cotinine, a 50% lower ratio of nicotine glucuronide to nicotine, and increased cotinine and trans-3′-hydroxycotinine. Nicotine equivalents, a robust biomarker of nicotine intake, were lower among Asp67Tyr heterozygotes compared with individuals without this allele: 58.2 (95% confidence interval, 48.9-68.2) versus 69.2 nmol/mL (95% confidence interval, 64.3-74.5). Conclusions: Individuals heterozygous for UGT2B10 Asp67Tyr consume less nicotine than do wild-type smokers. This striking observation suggests that variations in nicotine N-glucuronidation, as reported for nicotine C-oxidation, may influence smoking behavior. Impact: UGT2B10 genotype influences nicotine metabolism and should be taken into account when characterizing the role of nicotine metabolism on smoking.

AB - Background: Tobacco exposure is routinely assessed by quantifying nicotine metabolites in plasma or urine. On average, 80% of nicotine undergoes C-oxidation to cotinine. However, interindividual variation in nicotine glucuronidation is substantial, and glucuronidation accounts for from 0% to 40% of total nicotine metabolism. We report here the effect of a polymorphism in a UDP-glucuronsyltransferase, UGT2B10, on nicotine metabolism and consumption. Methods: Nicotine, cotinine, their N-glucuronide conjugates, and total trans-3′-hydroxycotinine were quantified in the urine (n = 327) and plasma (n = 115) of smokers. Urinary nicotine N-oxide was quantified in 105 smokers. Nicotine equivalents, the sum of nicotine and all major metabolites, were calculated for each smoker. The relationship of the UGT2B10 Asp67Tyr allele to nicotine equivalents, N-glucuronidation, and C-oxidation was determined. Results: Individuals heterozygous for the Asp67Tyr allele excreted less nicotine or cotinine as their glucuronide conjugates than did wild-type, resulting in a 60% lower ratio of cotinine glucuronide to cotinine, a 50% lower ratio of nicotine glucuronide to nicotine, and increased cotinine and trans-3′-hydroxycotinine. Nicotine equivalents, a robust biomarker of nicotine intake, were lower among Asp67Tyr heterozygotes compared with individuals without this allele: 58.2 (95% confidence interval, 48.9-68.2) versus 69.2 nmol/mL (95% confidence interval, 64.3-74.5). Conclusions: Individuals heterozygous for UGT2B10 Asp67Tyr consume less nicotine than do wild-type smokers. This striking observation suggests that variations in nicotine N-glucuronidation, as reported for nicotine C-oxidation, may influence smoking behavior. Impact: UGT2B10 genotype influences nicotine metabolism and should be taken into account when characterizing the role of nicotine metabolism on smoking.

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