Tylosin-resistant bacteria cultivated from agricultural soil

Leslie J. Onan, Timothy M. LaPara

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

In this study we analyzed the numbers and types of cultivable tylosin-resistant bacteria from six agricultural soils that differed with respect to their association with subtherapeutic antibiotic use. The proportion of tylosin-resistant bacteria to the total number of bacteria cultivated was significantly higher (7.2-16.5%) at three sites affected by subtherapeutic antibiotic use compared to three sites unaffected by subtherapeutic antibiotic use (0.7-2.5%). We also detected differences in the types of cultivable tylosin-resistant bacteria. At a site affected by subtherapeutic antibiotic use, we detected tylosin-resistant bacteria from the α- and β-subdivisions of Proteobacteria. In contrast, at a site unaffected by subtherapeutic use, we detected only Streptomyces-like (high-G+C Gram-positive) tylosin-resistant bacteria. Our results suggest a link between subtherapeutic use of antibiotics and the numbers and types of antibiotic-resistant bacteria in nearby soil. However, other factors, such as soil type and temporal variation, may have also contributed to the differences observed.

Original languageEnglish (US)
Pages (from-to)15-20
Number of pages6
JournalFEMS Microbiology Letters
Volume220
Issue number1
DOIs
StatePublished - Mar 14 2003

Bibliographical note

Funding Information:
We thank the numerous anonymous farmers who graciously allowed us to collect samples and Sara Firl for technical assistance. The University of Minnesota financially supported this research.

Keywords

  • Antibiotic
  • Growth promotion
  • Macrolide
  • Resistance

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