TXTL-based approach to synthetic cells

Jonathan Garamella, David Garenne, Vincent Noireaux

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

Cell-free transcription–translation (TXTL) has recently emerged as a versatile technology to engineer biological systems. In this chapter, we show how an all E. coli TXTL system can be used to build synthetic cell prototypes. We describe methods to encapsulate TXTL reactions in cell-sized liposomes, with an emphasis on the composition of the external solution and lipid bilayer. Cell-free expression is quantitatively described in bulk reactions and liposomes for three proteins: the soluble reporter protein eGFP, the membrane proteins alpha-hemolysin (AH) from Staphylococcus aureus, and the mechanosensitive channel of large conductance (MscL) from E. coli.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages217-239
Number of pages23
DOIs
StatePublished - 2019

Publication series

NameMethods in Enzymology
Volume617
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Bioengineering
  • Cell-free transcription–translation
  • Gene circuits
  • Liposomes
  • Membrane proteins
  • Synthetic biology
  • Synthetic cell

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

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