Two yeast PUF proteins negatively regulate a single mRNA

Brad A. Hook, Aaron C. Goldstrohm, Daniel J. Seay, Marvin Wickens

Research output: Contribution to journalArticlepeer-review

74 Scopus citations


mRNA stability and translation are regulated by protein repressors that bind 3′-untranslated regions. PUF proteins provide a paradigm for these regulatory molecules: like other repressors, they inhibit translation, enhance mRNA decay, and promote poly(A) removal. Here we show that a single mRNA in Saccharomyces cerevisiae, encoding the HO endonuclease, is regulated by two distinct PUF proteins, Puf4p and Mpt5p. These proteins bind to adjacent sites and can co-occupy the mRNA. Both proteins are required for full repression and deadenylation in vivo; their removal dramatically stabilizes the mRNA. The two proteins act through overlapping but non-identical mechanisms: repression by Puf4p is dependent on deadenylation, whereas repression by Mpt5p can occur through additional mechanisms. Combinatorial action of the two regulatory proteins may allow responses to specific environmental cues and be common in 3′-untranslated region-mediated control.

Original languageEnglish (US)
Pages (from-to)15430-15438
Number of pages9
JournalJournal of Biological Chemistry
Issue number21
StatePublished - May 25 2007


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