Two-pronged survival strategy for the major cystic fibrosis pathogen, Pseudomonas aeruginosa, lacking the capacity to degrade nitric oxide during anaerobic respiration

Sang Sun Yoon, Ahmet C. Karabulut, John D Lipscomb, Robert F. Hennigan, Sergei V. Lymar, Stephanie L. Groce, Andrew B. Herr, Michael L. Howell, Patricia J. Kiley, Michael J. Schurr, Benjamin Gaston, Kyoung Hee Choi, Herbert P. Schweizer, Daniel J. Hassett

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54 Scopus citations

Abstract

Protection from NO gas, a toxic byproduct of anaerobic respiration in Pseudomonas aeruginosa, is mediated by nitric oxide (NO) reductase (NOR), the norCB gene product. Nevertheless, a norCB mutant that accumulated ∼13.6 μM NO paradoxically survived anaerobic growth. Transcription of genes encoding nitrate and nitrite reductases, the enzymes responsible for NO production, was reduced ≥50- and 2.5-fold in the norCB mutant. This was due, in part, to a predicted compromise of the [4Fe-4S]2+ cluster in the anaerobic regulator ANR by physiological NO levels, resulting in an inability to bind to its cognate promoter DNA sequences. Remarkably, two O 2-dependent dioxygenases, homogentisate-1,2-dioxygenase (HmgA) and 4-hydroxyphenylpyruvate dioxygenase (Hpd), were derepressed in the norCB mutant. Electron paramagnetic resonance studies showed that HmgA and Hpd bound NO avidly, and helped protect the norCB mutant in anaerobic biofilms. These data suggest that protection of a P. aeruginosa norCB mutant against anaerobic NO toxicity occurs by both control of NO supply and reassignment of metabolic enzymes to the task of NO sequestration.

Original languageEnglish (US)
Pages (from-to)3662-3672
Number of pages11
JournalEMBO Journal
Volume26
Issue number15
DOIs
StatePublished - Aug 8 2007

Keywords

  • Anaerobic nitrate regulator (ANR)
  • Anaerobic respiration
  • Biofilms
  • Nitric oxide
  • Pseudomonas aeruginosa

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    Yoon, S. S., Karabulut, A. C., Lipscomb, J. D., Hennigan, R. F., Lymar, S. V., Groce, S. L., Herr, A. B., Howell, M. L., Kiley, P. J., Schurr, M. J., Gaston, B., Choi, K. H., Schweizer, H. P., & Hassett, D. J. (2007). Two-pronged survival strategy for the major cystic fibrosis pathogen, Pseudomonas aeruginosa, lacking the capacity to degrade nitric oxide during anaerobic respiration. EMBO Journal, 26(15), 3662-3672. https://doi.org/10.1038/sj.emboj.7601787