The clone 4921537P18 expressed preponderantly in mouse testis was identified by screening the Riken cDNA database, and two new full-length isoforms of this clone, which were named gsarp1 (Gonad Specific Ankyrin Repeat (ANK) Protein 1) and gsarp2, were found and isolated from mouse testis in the course of the research. Both of the GSARP1 and GSARP2 contain an ANK region circular composed by seven ANKs, and their structural feature is very similar to that of the IκB family proteins, while IκB proteins associate with the transcription factor NF-κB via their ANKs in the NF-κB pathway. We investigated the expression pattern at the mRNA level by Reverse transcription PCR. The gsarp1 has high expression level in mouse testis, while has low expression level in the ovary, and the gsarp2 is only expressed in mouse testis. The gsarp1 and gsarp2 begin to be detected at the early and later pachytene stage of meiosis separately, while both have high-expression level at the stage of MI and MII. The result of in situ hybridization reveals that the gsarp1 is primarily expressed in spermatocytes, while gsarp2 is expressed in spermatocytes and spermatids. In view of the structural feature and expression pattern of the GSARP1 and GSARP2, we speculate that they may play a certain role in a signal pathway of meiosis.
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Acknowledgments This work was supported by grants from the ICGEB International Center for Genetic Engineering and Biotechnology (ICGEB) (CRP/CHNO2-01), the National Basic Research Program of China (No. 2004CB117400), and the National Natural Science Foundation of China (No. 00510494; No. 30270675).
- Ankyrin repeat (ANK)
- Gonad specific expression
- In situ hybridization