Two novel proteins bind specifically to trichosanthin on choriocarcinoma cell membrane

Xuechun Xia, Fajian Hou, Jie Li, Yibao Ke, Huiling Nie

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Trichosanthin is the active protein component in the Chinese herb Trichosanthes kirilowi, which has distinct pharmacological properties. The cytotoxicity of trichosanthin was demonstrated by its selective inhibition of various choriocarcinoma cells. When Jar cells were treated with trichosanthin, the influx of calcium into the cells was observed by confocal laser scanning microscopy. When the distribution of trichosanthin-binding proteins on Jar cells was studied, two classes of binding sites for trichosanthin were shown by radioligand binding assay. Furthermore, the cytoplasmic membrane of Jar cells was biotinylated and the trichosanthin-binding proteins were isolated with trichosanthin-coupled Sepharose beads. Two protein bands with molecular masses of about 50 kDa and 60 kDa were revealed, further characterization of which should shed light on the mechanism of the selective cytotoxicity of trichosanthin to Jar cells.

Original languageEnglish (US)
Pages (from-to)725-731
Number of pages7
JournalJournal of Biochemistry
Volume139
Issue number4
DOIs
StatePublished - Apr 1 2006

Keywords

  • Cell surface biotinylation
  • Choriocarcinoma cells
  • Cytotoxicity
  • Radioligand binding assay
  • Trichosanthin

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