Two legume defense proteins suppress the mobility of nasopharyngeal carcinoma cells

Xiuli Dan, Tzi Bun Ng

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


A 16-kDa trypsin inhibitor was isolated from an edible legume using various chromatographic procedures. The protein was unadsorbed on Affi-gel blue gel but adsorbed on DEAE-Sepharose and Mono Q following which media the protein was subsequently subjected to gel filtration on Superdex 75 and a final 21-fold purification was achieved. This trypsin inhibitor showed remarkable pH and thermal stability. Its inhibitory activity was impaired in the presence of 1 mM dithiothreitol. The anti-proliferative and anti-mobility activities of this trypsin inhibitor and a hemagglutinin isolated from the same legume were tested on nasopharyngeal carcinoma cells. These two defense proteins demonstrated discrepant anti-proliferative efficacies that the hemagglutinin could greatly suppress the proliferation of nasopharyngeal carcinoma cells, while the trypsin inhibitor revealed a minor effect. However, these two proteins could both attenuate the mobility of nasopharyngeal carcinoma cells. The present study revealed the potential of applying plant defense proteins in cancer treatment.

Original languageEnglish (US)
Pages (from-to)1328-1334
Number of pages7
JournalJournal of Enzyme Inhibition and Medicinal Chemistry
Issue number6
StatePublished - Nov 1 2016
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2016 Informa UK Limited, trading as Taylor & Francis Group.


  • Hemagglutinin
  • legume defense proteins
  • nasopharyngeal carcinoma cells
  • trypsin inhibitor


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