Two enone fatty acids isolated from Gracilaria verrucosa suppress the production of inflammatory mediators by down-regulating NF-κB and STAT1 activity in lipopolysaccharide-stimulated RAW 264.7 cells

Hye Ja Lee, Hung The Dang, Gyeoung Jin Kang, Eun Jin Yang, Sun Soon Park, Weon Jong Yoon, Jee H. Jung, Hee Kyoung Kang, Eun Sook Yoo

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Gracilaria verrucosa is a common marine red alga that has anti-oxidant and anti-cancer properties. Recently, we reported that anti-inflammatory constituents of G. verrucosa operate through an unknown mechanism. For this reason, we isolated two enone fatty acids from G. verrucosa and investigated their molecular mechanism in LPS-stimulated RAW264.7 cells. We found that the two compounds inhibited the production of inflammatory markers (nitric oxide, TNF-α, and IL-6) in a dose-dependent manner. We next studied the effects of G. verrucosa compounds on LPS-induced signaling pathways. The two compounds suppressed NF-κB reporter activity by interfering with nuclear translocation of NF-κB and suppressed JAK/STAT (p-STAT1) signaling. These results suggest that G. verrucosa inhibits the production of inflammatory mediators (NO, TNF-α, and IL-6) by suppressing the activation of NF-κB and the phosphorylation of STAT1.

Original languageEnglish (US)
Pages (from-to)453-462
Number of pages10
JournalArchives of Pharmacal Research
Volume32
Issue number3
DOIs
StatePublished - Mar 2009

Bibliographical note

Funding Information:
This work was supported by Brain Korea 21 program of the Korea Research Foundation.

Keywords

  • Gracilaria verrucosa
  • Inflammation
  • NF-κB
  • Nitric oxide
  • Pro-inflammatory cytokines
  • STAT1

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