Two-dimensional culture systems to investigate mechanical interactions of the cell

Brett N. Napiwocki; Alana Stempien; Jacob Notbohm; Randolph S. Ashton; Wendy Crone

doi:10.1007/978-3-319-63552-1_6

Two-dimensional culture systems to investigate mechanical interactions of the cell

Brett N. Napiwocki, Alana Stempien, Jacob Notbohm, Randolph S. Ashton, Wendy Crone

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

Creation of well-controlled two dimensional cell culture systems continues to be important for the advancement of basic cell biology investigations as well as innovative platforms for drug development, toxicity testing, and diagnostic assays. These cell culture systems have become more advanced in order to provide the appropriate biomechanical and biochemical cues that better mimic the milieu of in vivo conditions. Additionally the platforms themselves can aid in the measurement of cell/cell and cell/substrate mechanical interactions through the use of microscopy techniques. Here we review state-of-the art cell culture systems that involve a range of different substrates, protein micropatterning, and markers for measurement of strains and tractions, including those developed by our labs. We will evaluate the advantages and disadvantages of several culture systems with a focus on those used with stem cell-derived cell types and provide examples of the quantitative measures that can be made with techniques such as traction force microscopy, digital image correlation, and scanning gradient Fourier transform analysis.

Original language	English (US)
Title of host publication	Mechanics of Biological Systems, Materials and other topics in Experimental and Applied Mechanics - Proceedings of the 2017 Annual Conference on Experimental and Applied Mechanics
Editors	Martha Grady, Wendy Crone, Pablo Zavattieri, Kathryn Dannemann, Majid Minary
Publisher	Springer New York LLC
Pages	37-39
Number of pages	3
ISBN (Print)	9783319635514
DOIs	https://doi.org/10.1007/978-3-319-63552-1_6
State	Published - 2018
Externally published	Yes
Event	Annual Conference and Exposition on Experimental and Applied Mechanics, 2017 - Indianapolis, United States Duration: Jun 12 2017 → Jun 15 2017

Publication series

Name	Conference Proceedings of the Society for Experimental Mechanics Series
Volume	4
ISSN (Print)	2191-5644
ISSN (Electronic)	2191-5652

Other

Other	Annual Conference and Exposition on Experimental and Applied Mechanics, 2017
Country/Territory	United States
City	Indianapolis
Period	6/12/17 → 6/15/17

Bibliographical note

Publisher Copyright:
© The Society for Experimental Mechanics, Inc. 2018.

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

Cell culture systems
Digital image correlation
Microcontact printing
Scanning gradient Fourier transform analysis
Traction force microscopy

Publisher link

10.1007/978-3-319-63552-1_6

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Cite this

Napiwocki, B. N., Stempien, A., Notbohm, J., Ashton, R. S., & Crone, W. (2018). Two-dimensional culture systems to investigate mechanical interactions of the cell. In M. Grady, W. Crone, P. Zavattieri, K. Dannemann, & M. Minary (Eds.), Mechanics of Biological Systems, Materials and other topics in Experimental and Applied Mechanics - Proceedings of the 2017 Annual Conference on Experimental and Applied Mechanics (pp. 37-39). (Conference Proceedings of the Society for Experimental Mechanics Series; Vol. 4). Springer New York LLC. https://doi.org/10.1007/978-3-319-63552-1_6

Two-dimensional culture systems to investigate mechanical interactions of the cell. / Napiwocki, Brett N.; Stempien, Alana; Notbohm, Jacob et al.
Mechanics of Biological Systems, Materials and other topics in Experimental and Applied Mechanics - Proceedings of the 2017 Annual Conference on Experimental and Applied Mechanics. ed. / Martha Grady; Wendy Crone; Pablo Zavattieri; Kathryn Dannemann; Majid Minary. Springer New York LLC, 2018. p. 37-39 (Conference Proceedings of the Society for Experimental Mechanics Series; Vol. 4).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Napiwocki, BN, Stempien, A, Notbohm, J, Ashton, RS & Crone, W 2018, Two-dimensional culture systems to investigate mechanical interactions of the cell. in M Grady, W Crone, P Zavattieri, K Dannemann & M Minary (eds), Mechanics of Biological Systems, Materials and other topics in Experimental and Applied Mechanics - Proceedings of the 2017 Annual Conference on Experimental and Applied Mechanics. Conference Proceedings of the Society for Experimental Mechanics Series, vol. 4, Springer New York LLC, pp. 37-39, Annual Conference and Exposition on Experimental and Applied Mechanics, 2017, Indianapolis, United States, 6/12/17. https://doi.org/10.1007/978-3-319-63552-1_6

Napiwocki BN, Stempien A, Notbohm J, Ashton RS, Crone W. Two-dimensional culture systems to investigate mechanical interactions of the cell. In Grady M, Crone W, Zavattieri P, Dannemann K, Minary M, editors, Mechanics of Biological Systems, Materials and other topics in Experimental and Applied Mechanics - Proceedings of the 2017 Annual Conference on Experimental and Applied Mechanics. Springer New York LLC. 2018. p. 37-39. (Conference Proceedings of the Society for Experimental Mechanics Series). doi: 10.1007/978-3-319-63552-1_6

Napiwocki, Brett N. ; Stempien, Alana ; Notbohm, Jacob et al. / Two-dimensional culture systems to investigate mechanical interactions of the cell. Mechanics of Biological Systems, Materials and other topics in Experimental and Applied Mechanics - Proceedings of the 2017 Annual Conference on Experimental and Applied Mechanics. editor / Martha Grady ; Wendy Crone ; Pablo Zavattieri ; Kathryn Dannemann ; Majid Minary. Springer New York LLC, 2018. pp. 37-39 (Conference Proceedings of the Society for Experimental Mechanics Series).

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Two-dimensional culture systems to investigate mechanical interactions of the cell

Abstract

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Keywords

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