Creation of well-controlled two dimensional cell culture systems continues to be important for the advancement of basic cell biology investigations as well as innovative platforms for drug development, toxicity testing, and diagnostic assays. These cell culture systems have become more advanced in order to provide the appropriate biomechanical and biochemical cues that better mimic the milieu of in vivo conditions. Additionally the platforms themselves can aid in the measurement of cell/cell and cell/substrate mechanical interactions through the use of microscopy techniques. Here we review state-of-the art cell culture systems that involve a range of different substrates, protein micropatterning, and markers for measurement of strains and tractions, including those developed by our labs. We will evaluate the advantages and disadvantages of several culture systems with a focus on those used with stem cell-derived cell types and provide examples of the quantitative measures that can be made with techniques such as traction force microscopy, digital image correlation, and scanning gradient Fourier transform analysis.
|Original language||English (US)|
|Title of host publication||Mechanics of Biological Systems, Materials and other topics in Experimental and Applied Mechanics - Proceedings of the 2017 Annual Conference on Experimental and Applied Mechanics|
|Editors||Martha Grady, Wendy Crone, Pablo Zavattieri, Kathryn Dannemann, Majid Minary|
|Publisher||Springer New York LLC|
|Number of pages||3|
|State||Published - 2018|
|Event||Annual Conference and Exposition on Experimental and Applied Mechanics, 2017 - Indianapolis, United States|
Duration: Jun 12 2017 → Jun 15 2017
|Name||Conference Proceedings of the Society for Experimental Mechanics Series|
|Other||Annual Conference and Exposition on Experimental and Applied Mechanics, 2017|
|Period||6/12/17 → 6/15/17|
Bibliographical notePublisher Copyright:
© The Society for Experimental Mechanics, Inc. 2018.
Copyright 2017 Elsevier B.V., All rights reserved.
- Cell culture systems
- Digital image correlation
- Microcontact printing
- Scanning gradient Fourier transform analysis
- Traction force microscopy