Two-dimensional culture systems to investigate mechanical interactions of the cell

Brett N. Napiwocki, Alana Stempien, Jacob Notbohm, Randolph S. Ashton, Wendy Crone

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations


Creation of well-controlled two dimensional cell culture systems continues to be important for the advancement of basic cell biology investigations as well as innovative platforms for drug development, toxicity testing, and diagnostic assays. These cell culture systems have become more advanced in order to provide the appropriate biomechanical and biochemical cues that better mimic the milieu of in vivo conditions. Additionally the platforms themselves can aid in the measurement of cell/cell and cell/substrate mechanical interactions through the use of microscopy techniques. Here we review state-of-the art cell culture systems that involve a range of different substrates, protein micropatterning, and markers for measurement of strains and tractions, including those developed by our labs. We will evaluate the advantages and disadvantages of several culture systems with a focus on those used with stem cell-derived cell types and provide examples of the quantitative measures that can be made with techniques such as traction force microscopy, digital image correlation, and scanning gradient Fourier transform analysis.

Original languageEnglish (US)
Title of host publicationMechanics of Biological Systems, Materials and other topics in Experimental and Applied Mechanics - Proceedings of the 2017 Annual Conference on Experimental and Applied Mechanics
EditorsMartha Grady, Wendy Crone, Pablo Zavattieri, Kathryn Dannemann, Majid Minary
PublisherSpringer New York LLC
Number of pages3
ISBN (Print)9783319635514
StatePublished - 2018
Externally publishedYes
EventAnnual Conference and Exposition on Experimental and Applied Mechanics, 2017 - Indianapolis, United States
Duration: Jun 12 2017Jun 15 2017

Publication series

NameConference Proceedings of the Society for Experimental Mechanics Series
ISSN (Print)2191-5644
ISSN (Electronic)2191-5652


OtherAnnual Conference and Exposition on Experimental and Applied Mechanics, 2017
Country/TerritoryUnited States

Bibliographical note

Publisher Copyright:
© The Society for Experimental Mechanics, Inc. 2018.

Copyright 2017 Elsevier B.V., All rights reserved.


  • Cell culture systems
  • Digital image correlation
  • Microcontact printing
  • Scanning gradient Fourier transform analysis
  • Traction force microscopy


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