Two critical N-terminal epitopes of the nucleocapsid protein contribute to the cross-reactivity between porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus

Wenting Xie, Chaojie Ao, Yilin Yang, Yinan Liu, Rui Liang, Zhe Zeng, Gang Ye, Shaobo Xiao, Zhen F. Fu, Wanyu Dong, Guiqing Peng

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Both porcine epidemic diarrhoea virus (PEDV) and porcine transmissible gastroenteritis virus (TGEV), which cause high mortality in piglets and produce similar clinical symptoms and histopathological morphology, belong to the genus Alphacoronavirus. Serological diagnosis plays an important role in distinguishing pathogen species. Together with the spike (S) protein, the nucleocapsid (N) protein is one of the immunodominant regions among coronaviruses. In this study, two-way antigenic cross-reactivity between the N proteins of PEDV and TGEV was observed by indirect immunofluorescence assay (IFA) and Western blot analysis. Furthermore, the PEDV N protein harbouring truncations of amino acids (aa) 1 to 170 or aa 125 to 301 was demonstrated to cross-react with the anti-TGEV N polyclonal antibody (PAb), whereas the truncation-expressing aa 302 to 401 resulted in a specific reaction with the anti-PEDV N PAb but not with the anti-TGEV N PAb. Mutants of the PEDV N protein were generated based on sequence alignment and structural analysis; we then confirmed that the N-terminal residues 58-RWRMRRGERIE-68 and 78-LGTGPHAD-85 contributed to the cross-reactivity. All the results provide vital clues for the development of precise diagnostic assays for porcine coronaviruses.

Original languageEnglish (US)
Article number001216
Pages (from-to)206-216
Number of pages11
JournalJournal of General Virology
Volume100
Issue number2
DOIs
StatePublished - Feb 2019
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by the National Key R&D Plan of China (programme no. 2018YFD0500100) and the Huazhong Agricultural University Scientific and Technological Self-Innovation Foundation (programme nos 2662015JQ003 and 2662017PY028).

Publisher Copyright:
© 2019 The Authors.

Keywords

  • Cross-reactivity
  • N-terminal epitopes
  • Nucleocapsid protein
  • PEDV
  • TGEV

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