TY - JOUR
T1 - Tumor suppressor p16 methylation in multiple myeloma
T2 - Biological and clinical implications
AU - Gonzalez-Paz, Natalia
AU - Chng, Wee J.
AU - McClure, Rebecca F.
AU - Blood, Emily
AU - Oken, Martin M.
AU - Van Ness, Brian
AU - James, C. David
AU - Kurtin, Paul J.
AU - Henderson, Kimberly
AU - Ahmann, Gregory J.
AU - Gertz, Morie
AU - Lacy, Martha
AU - Dispenzieri, Angela
AU - Greipp, Philip R.
AU - Fonseca, Rafael
PY - 2007/2/1
Y1 - 2007/2/1
N2 - The biological and clinical implications of p16 gene methylation in multiple myeloma (MM) are still unclear despite previous studies. In this comprehensive study, using methylation-specific PCR (MS-PCR), we show that p16 methylation is relatively common and occurs in monoclonal gammopathy of undetermined significance (MGUS; n = 17), smoldering multiple myeloma (SMM; n = 40), and MM (n = 522) at a prevalence of 24%, 28%, and 34%, respectively. However, p16 methylation does not appear to affect gene expression level. In a large cohort of patients with long-term follow-up information (n = 439), there was no difference in overall survival between patients with or without p16 methylation. We also found no association between p16 methylation and the main cytogenetic categories, although it was more common among patients with 17p13.1 deletions (p53 locus), a genetic progression event in MM. In addition, p16 methylation has no apparent effect on the cycle because there was also no difference in the plasma cell labeling index (a direct measurement of proliferation) between patients with and without p16 methylation. Our results question a major role for p16 methylation in the oncogenesis of the PC neoplasm, and we now believe p16 methylation may be a marker for overall epigenetic changes associated with disease progression, with no obvious direct biological or clinical consequences.
AB - The biological and clinical implications of p16 gene methylation in multiple myeloma (MM) are still unclear despite previous studies. In this comprehensive study, using methylation-specific PCR (MS-PCR), we show that p16 methylation is relatively common and occurs in monoclonal gammopathy of undetermined significance (MGUS; n = 17), smoldering multiple myeloma (SMM; n = 40), and MM (n = 522) at a prevalence of 24%, 28%, and 34%, respectively. However, p16 methylation does not appear to affect gene expression level. In a large cohort of patients with long-term follow-up information (n = 439), there was no difference in overall survival between patients with or without p16 methylation. We also found no association between p16 methylation and the main cytogenetic categories, although it was more common among patients with 17p13.1 deletions (p53 locus), a genetic progression event in MM. In addition, p16 methylation has no apparent effect on the cycle because there was also no difference in the plasma cell labeling index (a direct measurement of proliferation) between patients with and without p16 methylation. Our results question a major role for p16 methylation in the oncogenesis of the PC neoplasm, and we now believe p16 methylation may be a marker for overall epigenetic changes associated with disease progression, with no obvious direct biological or clinical consequences.
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U2 - 10.1182/blood-2006-05-024661
DO - 10.1182/blood-2006-05-024661
M3 - Article
C2 - 16840723
AN - SCOPUS:33846852215
SN - 0006-4971
VL - 109
SP - 1228
EP - 1232
JO - Blood
JF - Blood
IS - 3
ER -