Tumor prevention and antitumor immunity with heat shock protein 70 induced by 15-deoxy-Δ12,14-prostaglandin J2 in transgenic adenocarcinoma of mouse prostate cells

D. K. Vanaja, M. E. Grossmann, E. Celis, C. Y.F. Young

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

The biological modifier Δ12-prostaglandin J2 and related prostaglandins have been reported to have significant growth-inhibitory activity with induction of heat shock proteins (Hsps). Tumor-derived Hsps have been shown previously to elicit specific immunity to tumors from which they are isolated. In this study, 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2)-induced Hsp70 was purified from transgenic adenocarcinoma mouse prostate cells (TRAMP-C2). It was then tested for its ability to activate specific CTLs and induce protective immunity against prostate cancer in C57BL/6 mice. Treatment of cells with 8.0 μM 15d-PGJ2 for 24 h caused significant induction of Hsp70 expression. The yield of Hsp70 purified from 15d-PGJ2-treated cells was 4-5-fold higher when compared with untreated TRAMP-C2 cells. Vaccination of mice with Hsps isolated from TRAMP-C2 cells elicited tumor-specific CTLs and prevented the growth of TRAMP-C2 tumors. These results indicate that the induced heat shock proteins may have promising applications for antitumor, T-cell immunotherapy. In particular, these findings have important implications for the development of novel anticancer therapies aimed at promoting an immune response to prostate tumors.

Original languageEnglish (US)
Pages (from-to)4714-4718
Number of pages5
JournalCancer Research
Volume60
Issue number17
StatePublished - Sep 1 2000

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