Tumor necrosis factor alpha upregulates human microglial cell production of interleukin-10 in vitro

W. S. Sheng, S. Hu, F. H. Kravitz, P. K. Peterson, C. C. Chao

Research output: Contribution to journalArticlepeer-review

94 Scopus citations

Abstract

Interleukin (IL)-10 appears to play an important regulatory role in the systemic inflammatory response; however, production of IL-10 within the human central nervous system has not been described. Using cultures of human fetal microglial cells, the resident macrophages of the brain, we investigated the production and regulation of bioactive IL-10. Lipopolysaccharide stimulated acute release of tumor necrosis factor (TNF)-α (peak by 8 h) and delayed production of IL-10 (over a 48-h period) in microglial cell cultures. Treatment of microglial cell cultures with TNF-α and IL-6 resulted in a dose-dependent release of IL-10. These cytokines also induced expression of IL-10 mRNA. Treatment of microglial cell cultures with IL-10 markedly inhibited TNF-α and IL-6 production. These findings suggest that during inflammation within the brain, acute release of TNF-α and IL-6 by activated microglia could promote subsequent release of IL-10, which functions to minimize the potential neurotoxic effects of proinflammatory cytokines.

Original languageEnglish (US)
Pages (from-to)604-608
Number of pages5
JournalClinical and Diagnostic Laboratory Immunology
Volume2
Issue number5
DOIs
StatePublished - 1995

Fingerprint

Dive into the research topics of 'Tumor necrosis factor alpha upregulates human microglial cell production of interleukin-10 in vitro'. Together they form a unique fingerprint.

Cite this