Tumor necrosis factor-alpha induced enhancement of cryosurgery

Raghav Goel, Guilio F. Paciotti, John C. Bischof

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations


Local recurrence of cancer after cryosurgery is related to the inability to monitor and predict destruction of cancer (temperatures > -40°C) within an iceball. We previously reported that a cytokine adjuvant TNF-α could be used to achieve complete cancer destruction at the periphery of an iceball (0 to -40°C). This study is a further development of that work in which cryosurgery was performed using cryoprobes operating at temperatures > -40°C. LNCaP Pro 5 tumor grown in a dorsal skin fold chamber (DSFC) was frozen at -6°C after TNF-α incubation for 4 or 24 hours. Tumors grown in the hind limb were frozen with a probe tip temperature of -40°C, 4 or 24 hours after systemic injection with TNF-α. Both cryosurgery alone or TNF-α treatment alone caused only a minimal damage to the tumor tissue at the conditions used in the study. The combination of TNF-α and cryosurgery produced a significant damage to the tumor tissue in both the DSFC and the hind limb model system. This augmentation in cryoinjury was found to be time-dependent with 4-hour time period between the two treatments being more effective than 24-hour. These results suggests the possibility of cryotreatment at temperatures > -40°C with the administration of TNF-α.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
StatePublished - 2008
EventPhotonic Therapeutics and Diagnostics IV - San Jose, CA, United States
Duration: Jan 19 2008Jan 19 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherPhotonic Therapeutics and Diagnostics IV
Country/TerritoryUnited States
CitySan Jose, CA


  • Cancer
  • Cryosurgery
  • DSFC
  • Gold
  • LNCaP
  • Nanoparticle
  • Prostate
  • TNF-α


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