TY - JOUR
T1 - Tumor necrosis factor-α is a direct hepatocyte mitogen in the rat
AU - Beyer, H. S.
AU - Theologides, A.
PY - 1993/1/1
Y1 - 1993/1/1
N2 - We previously reported that tumor necrosis factor-α (TNF-α) increases in vivo hepatocyte mitoses and liver regeneration in rats. In the present studies, we used in vitro hepatocyte cultures to determine whether TNF-α itself was mitogenic or whether other cytokines (IL-1β and IL-6) that share similar actions with TNF-α might be involved. Hepatocytes were cultured with TNF-α (4, 40 or 400 U/ml), IL-1β (0.1, 1 and 10 ng/ml) or IL-6 (0.2, 2, or 20 ng/ml) for 24 h and 3 d. Incorporation of [3H]thymidine was increased significantly by TNF-α (40 and 400 U/ml) at 3 d. Both IL-1β and IL-6 at all concentrations significantly decreased [3H]thymidine incorporation at 3 d. These results indicate that TNF-α has a direct mitogenic action on hepatocytes. In contrast, IL-1β and IL-6 appear to suppress DNA synthesis in hepatocytes.
AB - We previously reported that tumor necrosis factor-α (TNF-α) increases in vivo hepatocyte mitoses and liver regeneration in rats. In the present studies, we used in vitro hepatocyte cultures to determine whether TNF-α itself was mitogenic or whether other cytokines (IL-1β and IL-6) that share similar actions with TNF-α might be involved. Hepatocytes were cultured with TNF-α (4, 40 or 400 U/ml), IL-1β (0.1, 1 and 10 ng/ml) or IL-6 (0.2, 2, or 20 ng/ml) for 24 h and 3 d. Incorporation of [3H]thymidine was increased significantly by TNF-α (40 and 400 U/ml) at 3 d. Both IL-1β and IL-6 at all concentrations significantly decreased [3H]thymidine incorporation at 3 d. These results indicate that TNF-α has a direct mitogenic action on hepatocytes. In contrast, IL-1β and IL-6 appear to suppress DNA synthesis in hepatocytes.
UR - http://www.scopus.com/inward/record.url?scp=0027511845&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027511845&partnerID=8YFLogxK
M3 - Article
C2 - 8490557
AN - SCOPUS:0027511845
SN - 1521-6543
VL - 29
SP - 1
EP - 4
JO - Biochemistry and Molecular Biology International
JF - Biochemistry and Molecular Biology International
IS - 1
ER -