Tumor necrosis factor-α differentially regulates the expression of proinflammatory genes in human airway smooth muscle cells by activation of interferon-β-dependent CD38 pathway

Omar Tliba, Reynold A. Panettieri, Samira Tliba, Timothy F. Walseth, Yassine Amrani

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Recent evidence suggests that CD38, an ectoenzyme that converts NAD + to cyclic ADP-ribose (cADPr), may play a role in cytokine-induced airway smooth muscle (ASM) cell hyper-responsiveness, a key feature associated with chronic asthma. In the present study, we investigated the major signaling pathways by which tumor necrosis factor-α (TNFα) induces CD38 expression and its role in regulating gene expression in human ASM cells. Using flow cytometry analyses, TNFα enhanced CD38 expression in a manner that was time-(0-24 h), concentration-(0.1-40 ng/ml), and protein synthesis-(cycloheximide blockade) dependent. A selective agonistic antibody against tumor necrosis factor receptor (TNFR) 1 also augmented CD38 expression, whereas anti-TNFR2 antagonistic antibody did not prevent the TNFα response. Inhibition of the Janus activated kinase/signal transducer and activator of transcription pathways using the soluble inhibitor 2-(1,1-dimethylethyl)-9-fluoro-3,6-dihydro-7H-benz-[h]imidaz[4,5-f] isoquinolin-7-one (DBI) or with neutralizing antibody against interferon β (IFNβ) completely abrogated TNFα-induced CD38 expression at both protein and mRNA levels. Combining TNFα (0.1 and 1 ng/ml) and IFNβ (100 IU/ml) at concentrations alone that had little effect on CD38 expression induced a robust synergistic induction of CD38 mRNA and protein levels. 8-Bromo-cADPr, a cADPr antagonist, significantly augmented TNFα-induced interleukin-6 secretion, whereas regulated on activation normal T cell expressed and secreted secretion was suppressed. 8-Bromo-cADPr, however, did not affect TNFα-induced cell surface expression of intercellular adhesion molecule-1. Our study is the first to demonstrate that IFNβ-dependent activation of CD38 pathway is a novel component by which TNFα differentially regulates the expression of inflammatory genes in ASM cells.

Original languageEnglish (US)
Pages (from-to)322-329
Number of pages8
JournalMolecular Pharmacology
Volume66
Issue number2
DOIs
StatePublished - Aug 1 2004

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