TROSY-based NMR experiments for the study of macromolecular dynamics and hydrogen bonding.

Guang Zhu, Youlin Xia, Donghai Lin, Xiaolian Gao

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Transverse relaxation-optimized spectroscopy (TROSY)-based nuclear magnetic resonance (NMR) experiments can be exploited to obtain chemical shift assignment and values of J-coupling constants, residual dipolar couplings, and nuclear Overhauser effects (NOEs) for structural studies of proteins, as discussed in Chapter 5. Furthermore, the application of TROSY-based NMR experiments can be extended to the measurements of molecule dynamics, amide proton exchange rates, and hydrogen bonds. This chapter describes these experiments.

Original languageEnglish (US)
Pages (from-to)161-184
Number of pages24
JournalMethods in molecular biology (Clifton, N.J.)
Volume278
StatePublished - 2004

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