TRH-like antidepressant peptide, pyroglutamyltyroslyprolineamide, occurs in rat brain

Eugene A. Pekary, Kym F. Faull, Mark Paulson, Robert L Lloyd, Albert Sattin

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


We have previously reported the occurrence of pGlu-Glu-Pro-NH2 (Glu-TRH, EEP), Val-TRH, Tyr-TRH, Leu-TRH, Phe-TRH, and Trp-TRH in rat brain using a combination of HPLC and radioimmunoassays with antibodies that cross-react with the general structure pGlu-X-Pro-NH2 where 'X' maybe any amino acid residue (Peptides 2004; 25:647). This new family of TRH-like peptides, along with TRH (pGlu-His-PrO-NH2), has neuroprotective, anticonvulsant, antidepressant, euphoric, anti-amnesic, and analeptic effects. We now report that a combination of affinity chromatography using a rabbit antibody specific for Tyr-TRH and Phe-TRH, along with HPLC and tandem mass spectrometry operating in the multiple reaction monitoring (MRM) mode, provide conclusive evidence for the presence of Tyr-TRH in rat brain. Furthermore, synthetic Tyr-TRH is active in the Porsolt Swim Test suggesting that it is a fourth member of this family of in vivo neuroregulatory agents that have psychopharmacotherapeutic properties.

Original languageEnglish (US)
Pages (from-to)1232-1236
Number of pages5
JournalJournal of Mass Spectrometry
Issue number9
StatePublished - Sep 2005


  • Affinity chromatography
  • HPLC
  • Mass spectrometry
  • TRH-like peptides


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