Treatment of oral candidiasis with methylene blue-mediated photodynamic therapy in an immunodeficient murine model

M. C. Teichert, J. W. Jones, M. N. Usacheva, M. A. Biel

Research output: Contribution to journalArticlepeer-review

188 Scopus citations


Objective. The purpose of this study was to evaluate the efficacy of using methylene blue (MB)-mediated photodynamic therapy to treat oral candidiasis in an immunosuppressed murine model, mimicking what is found in human patients. Study design. Seventy-five experimental mice with severe combined immunodeficiency disease were inoculated orally with Candida albicans by swab 3 times a week for a 4-week period. On treatment day, mice were cultured for baseline fungal growth and received a topical oral cavity administration of 0.05 ml_ MB solution at one of the following concentrations: 250, 275, 300, 350, 400, 450, or 500 μg/mL After 10 minutes the mice were recultured and underwent light activation with 664 nm of diode laser light with a cylindrical diffuser. After photodynamic therapy the mice were cultured again for colony-forming units per milliliter and then killed, their tissue harvested for histopathology. Results and conclusions. The results indicate an MB dose-dependent effect. Concentrations from 250 to 400 μg/mL reduced fungal growth but did not eliminate Candida albicans. MB concentrations of 450 and 500 μg/mL totally eradicated Candida albicans from the oral cavity, resulting in reductions from 2.5 log10 and 2.74 log10 to 0, respectively. These results suggest that MB-mediated photodynamic therapy can potentially be used to treat oral candidiasis in immunodeficient patients.

Original languageEnglish (US)
Pages (from-to)155-160
Number of pages6
JournalOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
Issue number2
StatePublished - 2002
Externally publishedYes

Bibliographical note

Funding Information:
Supported by the National Institutes of Health Grant #1R43AI/DE4186501.


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