Treatment of high salinity phenol-laden wastewater using a sequencing batch reactor containing halophilic bacterial community

Yu Feng Wang, Xiao Li Wang, Hui Li, Kuang Fei Lin, Ping Wang, Jie Yang, Yong Di Liu, Zhi Yuan Sun, Li Hua Fan, Zhi Ming Wu

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Sequencing batch reactors (SBRs) were used to degrade phenol compounds efficiently; however, most investigations on such reactors have focused on fresh rather than saline wastewaters, even though saline effluents containing phenolic compounds are generated by many industries. This study assessed the performance of the aerobic SBR process in the removal of phenol as the sole substrate under conditions of high salinity. A flexible concentration of phenol could be completely degraded in SBR process, varied from 400mgl-1 to 1200mgl-1 at the existing of 80gl-1 NaCl. The value of specific oxygen uptake rate (SOUR) suggested that the exiting bacteria could adapt to the phenol and salt conditions well at each stage. Cloning and sequencing of the 16S rRNA showed that the acclimated active sludge included two dominant genera: Pseudomonas and Alcaligenes. PCR detection of the functional genes suggested that phenol hydroxylase (Lph), catechol 1,2-dioxygenase (C12O), and catechol 2,3-dioxygenase (C23O) were active in the phenol-degradation process. Real-time PCR showed that the phenol-degrading bacteria comprised 63.3% of the total bacterial community.

Original languageEnglish (US)
Pages (from-to)138-144
Number of pages7
JournalInternational Biodeterioration and Biodegradation
Volume93
DOIs
StatePublished - Sep 2014

Keywords

  • Biodegradation
  • High-salinity
  • Moderate halophiles
  • Phenol-laden wastewater
  • Sequencing batch reactor

Fingerprint Dive into the research topics of 'Treatment of high salinity phenol-laden wastewater using a sequencing batch reactor containing halophilic bacterial community'. Together they form a unique fingerprint.

  • Cite this