Transposon-mediated mutagenesis in somatic cells: Identification of transposon-genomic DNA junctions

David A Largaespada, Lara S. Collier

Research output: Chapter in Book/Report/Conference proceedingChapter

27 Scopus citations

Abstract

Understanding the genetic basis for tumor formation is crucial for treating cancer. Forward genetic screens using insertional mutagenesis technologies have identified many important tumor suppressor genes and oncogenes in mouse models of human cancer. Traditionally, retroviruses have been used for this purpose, allowing the identification of genes that can cause various forms of leukemia or lymphoma with murine leukemia viruses or mammary cancer with mouse mammary tumor viruses. Recently, the Sleeping Beauty transposon system has emerged as a tool for cancer gene discovery in mouse models of human cancer. Transposons mobilized in the mouse soma can insertionally mutate cancer genes, and the transposon itself serves as a molecular "tag," which facilitates candidate cancer gene identification. We provide an overview of some general issues related to use of Sleeping Beauty for cancer genetic studies and present here the polymerase chain reaction-based method for cloning transposon-tagged sequences from tumors.

Original languageEnglish (US)
Title of host publicationChromosomal Mutagenesis
PublisherHumana Press
Pages95-108
Number of pages14
Volume435
ISBN (Print)9781588298997
DOIs
StatePublished - Apr 22 2008

Publication series

NameMethods in Molecular Biology
Volume435
ISSN (Print)1064-3745

Keywords

  • Cancer genetics
  • Linker-mediated PCR
  • Mouse transgenesis
  • Sleeping Beauty
  • Somatic mutagenesis
  • Transposon

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