Transport of 14C-deferiprone in normal, thalassaemic and sickle red blood cells

Oded Shalev, Dona Hileti, Philip Nortey, Robert P. Hebbel, Victor A. Hoffbrand

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The transport of deferiprone (L1) in normal (N), sickle (S) and thalassaemic (T) red blood cells (RBC) was determined by incubation with 14C-L1 at 37°C. Following incubation with 0.5 mM 14C-L1 for 4h, the intracellular concentration of L1 in T RBC was 3 times higher than was found extracellularly. In contrast, no concentration gradient across N and S RBC membranes was detected. Efflux studies showed that T RBC released only 17 ± 2% of 14C-L1 into the extracellular space. We hypothesize that L1 accumulation in T RBC results from their high content of chelatable iron and formation of large, hydrophilic L1-Fe(III) complexes trapped within the cytosol.

Original languageEnglish (US)
Pages (from-to)1081-1083
Number of pages3
JournalBritish journal of haematology
Volume105
Issue number4
DOIs
StatePublished - 1999

Keywords

  • Deferiprone
  • Erythrocytes
  • Sickle cell disease
  • Thalassaemia
  • Transport

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