Transport function of rice amino acid permeases (AAPs)

Margaret R. Taylor, Anke Reinders, John M. Ward

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


The transport function of four rice (Oryza sativa) amino acid permeases (AAPs), OsAAP1 (Os07g04180), OsAAP3 (Os06g36180), OsAAP7 (Os05g34980) and OsAAP16 (Os12g08090), was analyzed by expression in Xenopus laevis oocytes and electrophysiology. OsAAP1, OsAAP7 and OsAAP16 functioned, similarly to Arabidopsis AAPs, as general amino acid permeases. OsAAP3 had a distinct substrate specificity compared with other rice or Arabidopsis AAPs. OsAAP3 transported the basic amino acids lysine and arginine well but selected against aromatic amino acids. The transport of basic amino acids was further analyzed for OsAAP1 and OsAAP3, and the results support the transport of both neutral and positively charged forms of basic amino acids by the rice AAPs. Cellular localization using the tandem enhanced green fluorescent protein (EGFP)-red fluorescent protein (RFP) reporter pHusion showed that OsAAP1 and OsAAP3 localized to the plasma membrane after transient expression in onion epidermal cells or stable expression in Arabidopsis.

Original languageEnglish (US)
Pages (from-to)1355-1363
Number of pages9
JournalPlant and Cell Physiology
Issue number7
StatePublished - Jul 2015

Bibliographical note

Publisher Copyright:
© The Author 2015. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists.


  • Amino acid transporters
  • Electrophysiology
  • Rice AAPs
  • Xenopus oocytes


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