Transport capacity of median eminence: In vitro uptake of 3H-LRF1

Sharon S. Vaala, K. M. Knigge

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

In vitro, median eminence was found to take up 3H-LRF rapidly, reach equilibrium in approximately 30 min, and achieve an uptake (30 pg LRF/mg) twice that accumulated by anterior pituitary. Conditions which challenged cellular integrity(boiling, treatment with hypertonic saline, etc.) caused marked in-creases in uptake. Uptake was temperature-dependent and required oxygen and glucose, but was not noticeably ouabain-sensitive. The dissociation of 3H-LRF from median eminence occurred rapidly into media containing a 1, 000-fold greater concentration of unlabeled LRF. Estradiol and testosterone, but not corticosterone, significantly inhibited uptake. Unlabeled LRF and TRF, but not MIF, provided significant competition for the uptake of 3H-LRF.

Original languageEnglish (US)
Pages (from-to)147-157
Number of pages11
JournalNeuroendocrinology
Volume15
Issue number3-4
DOIs
StatePublished - Jan 1 1974

Keywords

  • Anterior pituitary
  • Hypothalamus
  • LRF
  • Median eminence

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